I am not aware of any such studies [dealing with specimen sizes] but it is a known fact since early XXth century that large specimens [such as complete brain slices] are known to be processed and yes, they require extended dehydrtaion → clearing → infiltration but not much more than usual IF the slices are thin enough, and yes, automated tissue processors can be used [I have done them for brain and whole prostates].René J.
On Thursday, April 9, 2015 2:26 PM, Caroline Miller <mi...@3scan.com> wrote: Hi there wonderful Histonet people, Has anyone seen any studies on the maximum size of tissue that can be paraffin (or resin) processed. I am not talking about the size that can fit in a tissue cassette, but, for example, entire pig bladder processing. I realize it would include extended processing time, temp, vacuum, agitation so imagine that none of these factors were limiting as I shall be using an automated tissue processor. I also realize that this is going to be tissue dependent. I was hoping there were already some studies in this area that I am not finding in both google and pubmed searches. thanks, in advance, for any links you can point me to, yours, Caroline -- Caroline Miller Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet On Thursday, April 9, 2015 2:26 PM, Caroline Miller <mi...@3scan.com> wrote: Hi there wonderful Histonet people, Has anyone seen any studies on the maximum size of tissue that can be paraffin (or resin) processed. I am not talking about the size that can fit in a tissue cassette, but, for example, entire pig bladder processing. I realize it would include extended processing time, temp, vacuum, agitation so imagine that none of these factors were limiting as I shall be using an automated tissue processor. I also realize that this is going to be tissue dependent. I was hoping there were already some studies in this area that I am not finding in both google and pubmed searches. thanks, in advance, for any links you can point me to, yours, Caroline -- Caroline Miller Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet