I had this issue a couple of years back. Found out that there was a delay of over an hour from the time the specimen was collected to the time it was being put into fixative.
Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roy, Lisa Sent: Tuesday, April 21, 2015 1:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nuclear "Artifact" Hi HistoNetters: I have run into quite a problem. My lab currently processes all tissue types from 3 different sites. Recently, we have been getting complaints from one of the sites that the biopsies have a nuclear "artifact". It is described as "washed out" or poor to no nuclear detail. Pictures have been uploaded (Nuclear "Artifact"). The Medical Director at said site is convinced that a processor error is occurring. Our site is not seeing this on any of our slides. Biopsies from all three sites are processed and embed together. We have done all trouble shooting that we can think of. Leica service has come to inspect our Peloris processor and all areas checked out as functioning properly. The problem is not consistent daily. Seems to be worst toward the end of the week. We have been running the same processing protocol, staining protocol and cutting protocols for years now. This problem has just developed over the last 2 months. Any ideas, no matter how far-fetched, would be greatly appreciated at this point. Lisa Roy, HT(ASCP) Histology Supervisor LabCorp at St. Vincent Hospital 123 Summer St Worcester, MA (508)363-9420 -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet