PTPM Acid is the bridge between the collagen and the aniline blue. It links the dye to the collagen. If you rinse the PTPM acid before putting the slide in the Aniline blue, you will not get the desired intensity of staining (of the collagen) that you are looking for. Cheers and Have a great week!
> Here is a message from Justine...> > From: Justine Lanzon [mailto:justinelan...@hotmail.com] > <justinelan...@hotmail.com]> > Sent: Thursday, March 05, 2015 5:36 AM > To: lindamarg...@gmail.com > Subject: Masson's trichrome stain > > > Hi, > > I am doing a write up on Masson's trichrome stain however I cannot > answer these two questions: > > - Why are plastic forceps used instead of metal ones to hold the > stained slide? > > - Why do we not rinse before Alinine blue? > > ? > > Can you please help me? > > ? > > Many Thanks, > > Justine Lanzon > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ We have literally about one hundred slides to re-slip for the this reason. Are there any suggestions for large numbers of slides to be re-coverslipped as this method would be too time consuming. We have used only glass for about nine years or so and it is much better. The old ones are the problem when someone needs "THAT" slide only. Pam Marcum UAMS -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N Sent: Wednesday, March 11, 2015 10:43 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Old slides Bernice, Take the slide and dip it in xylene. Lay it on the film, pressing down firmly. As it adheres, then gently wipe the excess xylene off, and gently place it in a book or your procedure manual and leave it there for an hour or so. Most of the bubbles will be gone, and the tissue will be saved. The original problem is not enough xylene dispersed onto the slide. Adjust the flow being dispensed by the unit. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 ---------------------------------------------------------------------- Message: 1 Date: Mon, 9 Mar 2015 19:41:48 +0000 From: Bernice Frederick <b-freder...@northwestern.edu> Subject: [Histonet] Old slides. To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <eb9d7062461640e5ac22d213a4acc...@evcspmbx03.ads.northwestern.edu> Content-Type: text/plain; charset="us-ascii" Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Hello, We received some liver tissue (Mouse) in 10% formalin (NFB). Then transferred to 70% ETOH. My question is that, Is it ok to transfer to 30% sucrose? So, frozen section can be perform. Please advise. Thanks, Bryan S. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, March 10, 2015 10:06 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 136, Issue 12 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Old slides. (Bernice Frederick) 2. RE: Old slides. (Jason McGough) 3. RE: Mushrooms for GMS fungus control (Morken, Timothy) 4. Re: FW: Masson's trichrome stain (John Kiernan) 5. RE: Old slides. (John Kiernan) 6. soft for microwriter (thermo scientific, Lamb, Shandon) (richard wild) 7. Re: Old slides. (b.curran.mcwill...@gmail.com) 8. Re: Old slides. (Rene J Buesa) 9. RE: Old slides. (Gowan,Christie C) 10. IHC / Morphometry Technician wanted in Shenandoah Valley Virginia (Erin Sarricks) ---------------------------------------------------------------------- Message: 1 Date: Mon, 9 Mar 2015 19:41:48 +0000 From: Bernice Frederick <b-freder...@northwestern.edu> Subject: [Histonet] Old slides. To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <eb9d7062461640e5ac22d213a4acc...@evcspmbx03.ads.northwestern.edu> Content-Type: text/plain; charset="us-ascii" Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> ------------------------------ Message: 2 Date: Mon, 9 Mar 2015 14:20:09 -0600 From: Jason McGough <jmcgo...@clinlab.com> Subject: RE: [Histonet] Old slides. To: Bernice Frederick <b-freder...@northwestern.edu>, histonet@lists.utsouthwestern.edu <histonet@lists.utsouthwestern.edu> Message-ID: <zarafa.54fe0079.5165.2525b65b03dde...@mail.clinlab.com> Content-Type: text/plain; charset=utf-8 Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgo...@clinlab.com <mailto:jmcgo...@clinlab.com> www.clinlab.com <http://www.clinlab.com> -----Original message----- > From:Bernice Frederick <b-freder...@northwestern.edu > <mailto:b-freder...@northwestern.edu> > > Sent: Monday, March 9, 2015 1:51 PM > To: histonet@lists.utsouthwestern.edu > <mailto:histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Old slides. > > Hi all, > We received some old slides (1997-1998) that were coverslipped with film. > Sakura I would imagine. The issue here is that the coverslips have come up > from the slide and the tissue is adhered to the back of the coverslip. They > need to be recovered so they can be evaluated. What do you all recommend? We > use the CV5030 for coverslipping. I tried one with xylene and mounting media > but there were still a couple of air bubbles in there. > Thanks, > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-freder...@northwestern.edu <mailto:b-freder...@northwestern.edu> > <mailto:b-freder...@northwestern.edu > <mailto:b-freder...@northwestern.edu> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > <mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > ------------------------------ Message: 3 Date: Mon, 9 Mar 2015 22:46:08 +0000 From: "Morken, Timothy" <timothy.mor...@ucsf.edu> Subject: [Histonet] RE: Mushrooms for GMS fungus control To: "koelli...@comcast.net" <koelli...@comcast.net> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <761e2b5697f795489c8710bcc72141ff367fb...@ex07.net.ucsf.edu> Content-Type: text/plain; charset="utf-8" Try this article... Acta Cytol. 2003 Nov-Dec;47(6):1043-4. Alternative, cost-effective fungus-staining method for control slides in cytology and histopathology. da Silva VD1. Author information Abstract OBJECTIVE: To develop a cost-effective, reliable and safe method of providing fungal control slides for routine use in pathology laboratories. STUDY DESIGN: A set of easily available, low-cost material was tested to obtain fungal colonies on substrate adequate for paraffin-embedded sections or smears. RESULTS: Such material as cheese is a simple, inexpensive and practical culture medium for silver-positive fungi. A batch of paraffin blocks can be prepared to maintain a stock of control material in the laboratory. CONCLUSION: It is useful to maintain fungal colonies to produce staining control specimens using small pieces of refrigerated cheese to easily produce silver-staining control specimens or smears embedded in paraffin, reducing the risk of accidental exposure to potentially infective pathogens in the laboratory. This method might also be a good alternative for conserving routine surgical specimens, considering the currently decreasing numbers of necropsy and large specimens, particularly from immunosuppressed and infected patients. PMID: 14674076 [PubMed - indexed for MEDLINE] -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of koelli...@comcast.net Sent: Sunday, March 08, 2015 4:29 PM To: Linda Prasad (SCHN) Cc: Jeffrey Robinson; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Mushrooms for GMS fungus control Apparently there are numerous interesting ways for fungus or bacteria controls to be had from orange peels to hamburger to slim Jim's to hot dogs to strawberries to ????.?? Sounds like fun to me.?? I'm curious, with the emphasis now on quality control in labs??run amok, has anyone passed a rigorous inspection actually showing these as your currently in-use controls??? A PI in research who??doesn't want??his paper rejected at peer review.?? A CAP inspector in clinical labs who is nit-picky reviewing staining controls but might be looking for a phase anything deficiency.?? The??dot-your-i's and cross-your-t's??FDA people who might or might not OK your drug in development.?? Really, just curious if anyone with a hammer over your head has said it is perfectly fine to use them. Ray, Seattle, WA ----- Original Message ----- From: "Linda Prasad (SCHN)" <linda.pra...@health.nsw.gov.au> To: "Jeffrey Robinson" <jrobin...@pathology-associates.com>, histonet@lists.utsouthwestern.edu Sent: Sunday, March 8, 2015 4:09:02 PM Subject: [Histonet] RE: Mushrooms for GMS fungus control I used strawberries for a fungal control. Worked really good. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.pra...@health.nsw.gov.au | w: www.schn.health.nsw.gov.au Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ???????Please consider the environment before printing this email. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Saturday, 7 March 2015 4:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mushrooms for GMS fungus control How about mushrooms? ??Has anyone had any success using mushrooms as a GMS fungus control? Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 10 Mar 2015 00:31:56 -0500 From: John Kiernan <jkier...@uwo.ca> Subject: Re: [Histonet] FW: Masson's trichrome stain To: Linda Margraf <lindamarg...@gmail.com>, histonet@lists.utsouthwestern.edu Cc: justinelan...@hotmail.com Message-ID: <7380eaed48941.54fe3...@uwo.ca> Content-Type: text/plain; charset=iso-8859-1 The notion of plastic forceps is new to me. Where did Justine find it? Nothing in any variant of the Masson procedure should be adversely affected by moving slides with stainless steel forceps. Is there a commercial campaign to sell plastic tweezers to Histonetters? John Kiernan = = = On 08/03/15, Linda Margraf <lindamarg...@gmail.com> wrote: > Here is a message from Justine... > > From: Justine Lanzon [mailto:justinelan...@hotmail.com] > <justinelan...@hotmail.com]> > Sent: Thursday, March 05, 2015 5:36 AM > To: lindamarg...@gmail.com > Subject: Masson's trichrome stain > > > Hi, > > I am doing a write up on Masson's trichrome stain however I cannot > answer these two questions: > > - Why are plastic forceps used instead of metal ones to hold the > stained slide? > > - Why do we not rinse before Alinine blue? > > ? > > Can you please help me? > > ? > > Many Thanks, > > Justine Lanzon > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 5 Date: Tue, 10 Mar 2015 00:40:02 -0500 From: John Kiernan <jkier...@uwo.ca> Subject: RE: [Histonet] Old slides. To: Jason McGough <jmcgo...@clinlab.com>, Bernice Frederick <b-freder...@northwestern.edu>, histonet@lists.utsouthwestern.edu, histonet@lists.utsouthwestern.edu Message-ID: <73e09aa74b442.54fe3...@uwo.ca> Content-Type: text/plain; charset=iso-8859-1 Have you done this? Acetone does not dissolve resinous mounting media and allow removal of coverslips. It's all in the books; buy one. John Kiernan Anatomy & Cell Biology, UWO London, Canada = = = On 09/03/15, Jason McGough <jmcgo...@clinlab.com> wrote: > Remove the film coverslip by placing the slide in acetone for a few minutes. > Then recoverslip the slide with your current method. > > > > Jason McGough, HT(ASCP) > > Operations Manager > > Clinical Laboratory of the Black Hills > > 605-343-2267 > > jmcgo...@clinlab.com <mailto:jmcgo...@clinlab.com > <jmcgo...@clinlab.com>> > > www.clinlab.com <http://www.clinlab.com> > > ? > ? > -----Original message----- > > From:Bernice Frederick <b-freder...@northwestern.edu > > <mailto:b-freder...@northwestern.edu <b-freder...@northwestern.edu>> > > > > > Sent: Monday, March 9, 2015 1:51 PM > > To: histonet@lists.utsouthwestern.edu > > <mailto:histonet@lists.utsouthwestern.edu > > <histonet@lists.utsouthwestern.edu>> > > Subject: [Histonet] Old slides. > > > > Hi all, > > We received some old slides (1997-1998) that were coverslipped with film. > > Sakura I would imagine. The issue here is that the coverslips have come up > > from the slide and the tissue is adhered to the back of the coverslip. They > > need to be recovered so they can be evaluated. What do you all recommend? > > We use the CV5030 for coverslipping. I tried one with xylene and mounting > > media but there were still a couple of air bubbles in there. > > Thanks, > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > b-freder...@northwestern.edu <mailto:b-freder...@northwestern.edu > > <b-freder...@northwestern.edu>> <mailto:b-freder...@northwestern.edu > > <b-freder...@northwestern.edu> <mailto:b-freder...@northwestern.edu > > <b-freder...@northwestern.edu>> > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > <mailto:Histonet@lists.utsouthwestern.edu > > <Histonet@lists.utsouthwestern.edu>> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 6 Date: Tue, 10 Mar 2015 10:05:11 +0100 From: richard wild <richard.w...@wanadoo.fr> Subject: [Histonet] soft for microwriter (thermo scientific, Lamb, Shandon) To: histonet@lists.utsouthwestern.edu Message-ID: <54feb3c7.40...@wanadoo.fr> Content-Type: text/plain; charset=utf-8; format=flowed Hi I am looking for labelling software (or advices) for the carousel microwriter (thermo scientific, Lamb, Shandon = same machine) (LAMB E22.01MWR) The machine is discontinuated. I would like to use serial interface rs232 and barcode scanners. Thanks for help. Richard ------------------------------ Message: 7 Date: Tue, 10 Mar 2015 11:51:25 +0000 From: b.curran.mcwill...@gmail.com Subject: Re: [Histonet] Old slides. To: Bernice Frederick <b-freder...@northwestern.edu> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <9fa4b5fb-11be-47c7-aa0c-6f8416b10...@gmail.com> Content-Type: text/plain; charset=us-ascii Hi We re-Coverslipper a number of sections which had peeled off on the tape as the tape dried and curled. We cut off excess tape using scissors; placed a fresh coverslip flat; put a streak of mounting medium on he coverslip; use a forceps to orientate the tissue + margin of tape; number a slide, dip it in Xylene & place on a slope & bring on top of coverslip-section-mounting medium; turn the slide-section- coverslip to face coverslip up; leave horizontal to dry (eg overnight). Worth a try, doing one first. Bernie, St Vincent's, Dublin, Ireland > On 9 Mar 2015, at 19:41, Bernice Frederick <b-freder...@northwestern.edu> > wrote: > > Hi all, > We received some old slides (1997-1998) that were coverslipped with film. > Sakura I would imagine. The issue here is that the coverslips have come up > from the slide and the tissue is adhered to the back of the coverslip. They > need to be recovered so they can be evaluated. What do you all recommend? We > use the CV5030 for coverslipping. I tried one with xylene and mounting media > but there were still a couple of air bubbles in there. > Thanks, > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 10 Mar 2015 13:43:35 +0000 (UTC) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Old slides. To: John Kiernan <jkier...@uwo.ca>, Jason McGough <jmcgo...@clinlab.com>, Bernice Frederick <b-freder...@northwestern.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1897656965.1753669.1425995015431.javamail.ya...@mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 To John Probably the writer is referring to slides "mounted" with the Sakura film??"coverslip".I have done it many times and the FILM will??detach easily from the section.Had it been a glass coverslip attached with, for example, Permount, acetone would not have done anything.Ren?? J.?? On Tuesday, March 10, 2015 1:40 AM, John Kiernan <jkier...@uwo.ca> wrote: Have you done this? Acetone does not dissolve resinous mounting media and allow removal of coverslips. It's all in the books; buy one. John Kiernan Anatomy & Cell Biology, UWO London, Canada = = = On 09/03/15, Jason McGough?? <jmcgo...@clinlab.com> wrote: > Remove the film coverslip by placing the slide in acetone for a few minutes. > Then recoverslip the slide with your current method. > > > > Jason McGough, HT(ASCP) > > Operations Manager > > Clinical Laboratory of the Black Hills > > 605-343-2267 > > jmcgo...@clinlab.com <mailto:jmcgo...@clinlab.com <jmcgo...@clinlab.com>> > > www.clinlab.com <http://www.clinlab.com> > > ?? > ?? > -----Original message----- > > From:Bernice Frederick <b-freder...@northwestern.edu > > <mailto:b-freder...@northwestern.edu <b-freder...@northwestern.edu>> > > > Sent: Monday, March 9, 2015 1:51 PM > > To: histonet@lists.utsouthwestern.edu > > <mailto:histonet@lists.utsouthwestern.edu > > <histonet@lists.utsouthwestern.edu>> > > Subject: [Histonet] Old slides. > > > > Hi all, > > We received some old slides (1997-1998) that were coverslipped with film. > > Sakura I would imagine. The issue here is that the coverslips have come up > > from the slide and the tissue is adhered to the back of the coverslip. They > > need to be recovered so they can be evaluated. What do you all recommend? > > We use the CV5030 for coverslipping. I tried one with xylene and mounting > > media but there were still a couple of air bubbles in there. > > Thanks, > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > b-freder...@northwestern.edu <mailto:b-freder...@northwestern.edu > > <b-freder...@northwestern.edu>> <mailto:b-freder...@northwestern.edu > > <b-freder...@northwestern.edu> <mailto:b-freder...@northwestern.edu > > <b-freder...@northwestern.edu>> > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu <mailto:Histonet@lists.utsouthwestern.edu > > <Histonet@lists.utsouthwestern.edu>> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 9 Mar 2015 20:01:10 +0000 From: "Gowan,Christie C" <christiecgo...@dermatology.med.ufl.edu> Subject: [Histonet] RE: Old slides. To: "'Bernice Frederick'" <b-freder...@northwestern.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <de45f62de02d4c3389de0af3f45e1...@ahc-exch03.ad.ufl.edu> Content-Type: text/plain; charset="us-ascii" Hi Bernice, I have found that if you flood the slide with mounting media (don't use xylene) flip the slide over onto an absorbent lab wipe and put a heavy weight with even pressure and leave for a few hours. If the slide sticks to the wipe just put a few drops of xylene to clean up the slide. You may still have some tiny bubbles but it is much better than the alternative. Good luck. Christie Gowan -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, March 09, 2015 3:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Old slides. Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 10 Mar 2015 12:12:05 -0400 From: Erin Sarricks <esarri...@gmail.com> Subject: [Histonet] IHC / Morphometry Technician wanted in Shenandoah Valley Virginia To: histonet <histonet@lists.utsouthwestern.edu> Message-ID: <CAPapK_1E+KJTXn9pLbWgLsVrd98Fdi1=ulpmhsbkssdb8nr...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Histology Laboratory located in the Shenandoah Valley of Virginia is looking to add to it's team. In this position, you will need a working knowledge of IHC theory and practical IHC experience. The best candidate for the position will oversee immunohistochemical staining as well as perform other histology functions including trimming of specimens, paraffin embedding, microtomy and microscopic QC of slides. Experience with morphometry is preferable. Desirable candidates will possess the following: - HT (ASCP) or QIHC registration preferred - 4 years of Histology experience - 1+ years of immunohistochemistry and/or immunofluorescence experience - Keeps abreast with company's current policies and immunohistochemistry technical updates and procedures - Must be able to work independently and in a team environment Full time employment benefits include subsidized medical and dental insurance, vacation, holiday pay, and 401k after 1 year of employment. Compensation is commensurate with experience. If you are interested in this position, please respond to this post with your resume and cover letter. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 136, Issue 12 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
