Hi!
I'm trying to produce paraffin sections showing intestinal bacteria in
intestinal tissue sections. We have tried routine PFA fixation and
paraffin processing, and a Carnoy-based protocol supposedly optimized
for this purpose. The problem is, the bacteria seem to shrink so much
that I can't see any nice shapes but only very tiny roughly circular
objects. What to do?
With best regards,
Mikael Niku, University of Helsinki
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