I Garrey, I came across floating sections in dilute alcohol back in 2005 when I started working in the UK and have been using it ever since. I agree it introduces one more step and wouldn't use for every single section - there's no need. But I do find it very helpful with certain blocks/tissues - especially the very 'wrinkly' ones.
When I started cutting during my training, we used other technique to avoid wrinkles (and waste of tissue sections) and produce 'perfect' sections. We used to have a small cold water bath to float and pick up the sections before transferring to the temperature controlled water bath. Joana Joana Moreira Supervisor - Anatomical Pathology Department of Pathology Sidra Medical & Research Center PO Box 26999 | Doha, Qatar Direct Line +974-4404-2036 jmore...@sidra.org | www.sidra.org Message: 12 Date: Sat, 6 Jun 2015 10:29:03 -0400 From: Garrey Faller <garr...@gmail.com> To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pre-floating tissue sections in dilute alcohol Message-ID: <a7285ef9-fa0f-4121-aa4c-78dfa5f1c...@gmail.com> Content-Type: text/plain; charset=us-ascii Hi everyone, I just became aware of this technique last week, and it seems to work great. I did a quick google search and found this quick reference. http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf <http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf> Anyone out there float their sections in dilute ethyl alcohol before transferring to the water bath? Its an extra step and introduces an extra chance to introduce floaters. But, the quality seems to be improved. Any thoughts? Thanks in advance. Garrey Faller Pathologist ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 139, Issue 6 **************************************** Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
