Some of our histotechs use just cold tapwater for wrinkled sections before transferring them into the warm waterbath. I don't know, if the added ethanol makes any difference (maybe reducing surface tension).
I would offer the possibility of using the additional waterbath, but let the histotechs decide, if it is necessary to use it. Gudrun Lang Leading histotech, Linz Austria -----Ursprüngliche Nachricht----- Von: Garrey Faller [mailto:garr...@gmail.com] Gesendet: Samstag, 06. Juni 2015 16:29 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Pre-floating tissue sections in dilute alcohol Hi everyone, I just became aware of this technique last week, and it seems to work great. I did a quick google search and found this quick reference. http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf <http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf> Anyone out there float their sections in dilute ethyl alcohol before transferring to the water bath? Its an extra step and introduces an extra chance to introduce floaters. But, the quality seems to be improved. Any thoughts? Thanks in advance. Garrey Faller Pathologist _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
