Instead of an antibody, you might consider enzyme activity histochemistry, which is much less expensive. Demonstration of 5-nucleotidase activity in the presence of levamisole detects lymphatic endothelium. Sections can also be stained for alkaline phosphatase activity in the endothelium of blood vessels. Here are a few references.
Kato, S., Yasunaga, A. and Uchida, U. (1991). Enzyme-histochemical method for identification of lymphatic capillaries. Lymphology 24:125-129. Ohkuma, M. (1994). Simultaneous double staining for the blood and lymphatic capillary. Lymphology 27, Suppl:106-107. Okada, E. (1994). An improved enzyme-histochemical method for identification of lymphatic capillaries on paraffin sections. Lymphology 27, Suppl:732-735. Ji, R.C. and Kato, S. (2003). Lymphatic network and lymphangiogenesis in the gastric wall. Journal of Histochemistry and Cytochemistry 51:331-338. Needless to say, none of these relate to amphibian tissues! John Kiernan Anatomy, UWO, London, Canada = = = On 26/07/15, Jason Palmer via Histonet <histonet@lists.utsouthwestern.edu> wrote: > > Hi all, > > I need to find an antibody that will label lymphatic endothelial cells in the > axolotl. Does anybody have any experience or ideas? I have tried a couple of > our anti-mouse and anti-human Abs for podoplanin and LYVE-1 but no > cross-reactivity so far. I have no experience with staining of non-mammalian > tissues - maybe an anti-frog Ab would cross react? Does anyone have > experience with other amphibians? > > Thanks, > > Jason > > -- > > Jason Palmer > Histology Laboratory Coordinator > O'Brien Institute / St Vincent's Institute > 42 Fitzroy St, Fitzroy Victoria 3065 > Australia > tel +61 3 9288 4045 > fax +61 3 9416 0926 > email: jpal...@svi.edu.au > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
