Hello all,
Has anyone using Richard Allen Hematoxylin-2 noticed an odd
artifact on the slides after using the Hematoxylin for more than a few days on
their stainer? We are seeing small spore or pollen-like blue dots here and
there on the slides. It is not coming from the water bath or our water supply
on the stainer. I used sterile gloves, opened a new case of slides, dipped them
in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI again,
air-dried and coverslipped them, and the blue dots were there. The only way we
got rid of the blue artifact was to use new RA Hematoxylin-2 every 2-3 days,
which is a bit expensive.
Thanks for your input, and if you can recommend a different,
reasonably priced hematoxylin, that would be awesome.
Cheers,
Sandy
Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine
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