Wow, I agree with Liz. There should not routinely be "so much tissue washing off". There is a fundamental problem, if this is the case.
With regards to hematoxylin, have you tried Gill's Hematoxylin, 1, 2, or 3? These do not need filtering and do not produce a precipitate. Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_man...@merck.com -----Original Message----- From: Elizabeth Chlipala via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 1:55 PM To: Tim H; 'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu) Subject: Re: [Histonet] Hematoxylin Precipitate I think you may a have a serious problem if "so much tissue washes off" if that is happening you have problems with tissue adherence. A properly processed and cut section should not wash off the slide, or even a portion of it should not wash off. That would mean that you did not provide to the pathologist what is represented in the block. If that is the case then you would need to filter all solutions on a stainer daily not just the hematoxylin. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Tim H via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Hematoxylin Precipitate You should be filtering your Hematoxylin on a daily basis regardless of what the manufactures says. We use to filter twice a day since we did a traditional overnight run and then again in the afternoon for specimens that had been microwave processed. So much tissue washes off in the solutions they should be changed or filtered fairly regularly to try and prevent cross contamination on the slides. You can also try increasing your rinse times and see if that doesn't help as well. Thanks, Tim > > Message: 1 > Date: Mon, 21 Sep 2015 15:14:39 -0500 > From: "Sandra Cheasty" <cheas...@svm.vetmed.wisc.edu> > To: "Histonet (histonet@lists.utsouthwestern.edu)" > <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Hematoxylin Precipitate > Message-ID: <4cda87133587e64c965ce6c356d18...@svm.vetmed.wisc.edu> > Content-Type: text/plain; charset="us-ascii" > > Hello all, > > Has anyone using Richard Allen Hematoxylin-2 noticed an odd > artifact on the slides after using the Hematoxylin for more than a few days > on their stainer? We are seeing small spore or pollen-like blue dots here and > there on the slides. It is not coming from the water bath or our water supply > on the stainer. I used sterile gloves, opened a new case of slides, dipped > them in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI > again, air-dried and coverslipped them, and the blue dots were there. The > only way we got rid of the blue artifact was to use new RA Hematoxylin-2 > every 2-3 days, which is a bit expensive. > > Thanks for your input, and if you can recommend a different, > reasonably priced hematoxylin, that would be awesome. > > Cheers, > > Sandy > > > > Sandra J. Cheasty, HT (ASCP) > > Histology & Necropsy Supervisor > > UW-Madison, School of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
