Hello, I’ve been used many different AMM's (from many companies). In my experience lab-made AMM is the best, there is *modified method *in ihcworld.com. I’m using this method; slides are good even one year past. And no bubbles.
On Wed, Nov 4, 2015 at 4:08 PM, Keyser Gerald T via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Currently my lab is using Aqua-mount (Lerner Laboratories). I'm looking at > replacing it with a different Aqueous mounting media. The only stain we use > it for is Oil Red O. > > I've tried many different variations on its use and the results have been > the same. > > 1) The slide looks lovely the day that the coverslip is mounted and > then dozens of bubbles form after. Sealing does not make a difference. > Following company recommended procedure doesn't make a difference. Getting > creative doesn't make a difference. Nothing seems to make a difference. > > 2) We use a non-aqueous media surrounding the aqueous and > post-coverslip the slide. This option is messy and cannot be > re-coverslipped (for obvious reasons) if there is an air bubble. I'm sure > that this wouldn't work at all with a more delicate stain. > > Ok. I give up. Can anyone make a recommendation for a different aqueous > mounting media? > > Gerry > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Asst. Prof. Dr. Mehmet Fatih BOZKURT Department of Pathology Faculty of Veterinary Medicine Afyon Kocatepe University 03100, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet