Dear Histonet Colleagues, I hope the holiday season has been good to all.
We are interested in a routinely used protocol to decal, process and paraffin embed mouse femur and tibia. Some of our projects may require a gentle decal, whereas others may not be so delicate. Also, we do have access to a microwave for decalcifying, if recommended to be a better method. It would certainly save a lot of time. It would be greatly appreciated if anyone would be willing to share a protocol for post-harvest sample storage (i.e. PBS, 70% ETOH, saline, etc.), decalcification, processing and embedding. Thank you in advance for your help!! Jim Herrick, M.S.A. Supervisor - Sr. Res.Tech. Department of Orthopedics Biomaterials and Histomorphometry Core Lab Biomaterials and Tissue Engineering Lab Office Phone: (507) 538-4300 Lab Phone: (507) 255-5946 Pager: 127 (13239) Fax: (507) 266-9451 Email: herrick.ja...@mayo.edu<mailto:herrick.ja...@mayo.edu> ______________________ Mayo Clinic 200 First Street SW Rochester, MN 55905 www.mayoclinic.org<http://www.mayoclinic.org/> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
