Hi Charles - Not to worry. Many of us Histo folks don't have a Cytologist to help. Beth is spot on in her advice. I just wanted to add Orange G should appear yellow to orange; 15 sec to 1 minute is the usual range of staining times. EA is often problematic because of fundamental limitations in its chemical composition. Ideally, one should see clearcut hues of green and red in separate cells. Staining times less than about 3 minutes usually favor the uptake of eosin, with eosin and light green often occupying different areas of the same cells. Most EA formulations perform optimally in the 6-8 minute range. Note particularly that the OG and EA staining times are interdependent: relatively too much time in OG will overload cells with orange G and block the subsequent uptake of eosin. Make sure you record your lots of stain as they are changed out, and try using a self-made buccal smear to check new lots of of the stain components before they are put into use. Then, if you see a te st problem, you can repeat the buccal smear and compare to the original. It may help you to pinpoint the problem. Best of luck - Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874
9. PAP staining quality (Beth Cox) Message: 9 Date: Thu, 4 Feb 2016 18:06:53 -0500 From: Beth Cox <bethc...@gmail.com> Subject: [Histonet] PAP staining quality Hi Charles, A couple things to check on: 1. The first concern I would have is your EA stain. Poor EA staining will give too much orange staining and pale other counterstains (making them look aged). What brand are you using? Have you changed brands? Is your EA close to the expiration date? Is the bulk stored with light exposure? I think fixing your EA will fix all the other problems. 2. The other question I have regards your alcohol. Have you changed types/brands? Pap staining is very delicate and the different alcohols used can make a big difference. Beth Cox, HTL/SCT(ASCP)QIHC ------------------------------ Message: 3 Date: Thu, 4 Feb 2016 09:50:18 -0500 From: Charles Riley <cri...@dpspa.com> Subject: [Histonet] PAP stain quality Not sure if anyone out the would know the answer to this. We are having an issue with our PAP stained slides appearing too orange and look aged. If you have any idea for causes I appreciate any help -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
