NSH has an archived webinar: Nuclear Bubbling: It's About Time" created by Peggy Wenk. The webinar talks about several causes of nuclear bubbling, changes that can be made to reduce this problem, and, for really bad cases, Peggy talks about how to dry the slide differently, reprocess tissue or use a HIER technique to improve the quality of the nuclear detail.
It's available to members through our online community: The Block: theblock.nsh.org Aubrey M.J. Wanner National Society for Histotechnology -----Original Message----- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Wednesday, February 17, 2016 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 147, Issue 16 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Sakura Pre-Processing Fixative (7117) for X120 Processors (Jonathan Jennings) 2. Re: Nuclear Bubbling (Rene J Buesa) 3. (CLRW) Clinical Laboratory Reagent Water (Brent Adams) 4. Re: Nuclear Bubbling (Jennifer MacDonald) 5. Re: Nuclear Bubbling (Manfre, Philip) 6. Re: Nuclear Bubbling (Jamal Rowaihi) 7. Re: Nuclear Bubbling (Vickroy, James) 8. nuclear bubbling (Carlos Defeo) 9. Re: Nuclear Bubbling (Katie Sands) 10. Sequenza Immunostaining Racks (Carlos Genty) 11. Error Prevention: (Jb) ---------------------------------------------------------------------- Message: 1 Date: Tue, 16 Feb 2016 18:00:59 +0000 From: Jonathan Jennings <jjenni...@thedermlab.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Sakura Pre-Processing Fixative (7117) for X120 Processors Message-ID: <248395418C8C074A83C7D06081C4F01634741ADB@DERMLAB-SBS.dermlab.local> Content-Type: text/plain; charset="us-ascii" Hello All, I manage a histology Dermpath lab (We only process skins) and we use Sakura X120 Processors. We have been doing excellent with the use of our x120 processors that allowed use to have slides on the Pathologist desk within 5 hours from the time we received the specimens. That said, there is always room for improvement! As anyone who uses the Sakura x120 Xpress processors know, all of the solutions and reagents required are "proprietary" so I don't know exactly what is in them. To top that off, we bought the processors from a 3rd party vendor, so Sakura isn't too quick to come out and help me with this. All of That noted, the reason I am sending this email is because I want to incorporate the pre-processing fixative because the specimens we receive in the afternoon haven't been in formalin as long (3-5 hours) as the specimens that we receive by mail in the morning (in formalin for 18+ hours). Our preprocessing solution is working ok, but I read in the user manual about preproces sing fixative that should be used instead, if the specimens were not completely fixed before processing. Here are a few details of our current processing workflow with only using preprocessing solution. * When grossing we have 3 small stirring hot plates set at 38 Celsius. Each stir plate described below o 1 for the 0.2 or < Biopsies filled with 10% NBF prior to preprocessing solution for 15 minutes then processed on standard (1 hour program) o 1 for the 0.3 or > Biopsies Filled with Statfix (statlab) Must be in statfix. Cassettes must be in for at least 30 minutes prior to preprocessing solution (30 minutes) Then Placed on the processor (Extended - 2 hour program) o 1 for the excisions, Cyst and all other fatty skin specimens, filled with statfix Cassettes must be in for at least 1 hour prior to preprocessing solution (30 minutes) The placed on processor (Extended - 2 hour program) I tried the preprocessing fixative (no heat, just stirring agitation) on test excisions and it definitely made the tissue more firm, (almost too hard) for 30 minutes. We had to use preprocessing solution for 1 hour on excisions, so that is a 30 minute improvement. It also turned the tissue yellow, which was weird. Before I try to test more tissue, I wanted to see if anyone else has went through the same preprocessing fixative testing with skins. Questions Finally! ;) * Does anyone use the preprocessing fixative for skin specimens and would you mind giving me some insight/advice on how to incorporate? * Is it ok to incorporate any heat to the preprocessing solution or preprocessing fixative to speed up processing time? * Does the preprocessing fixative affect IHCs any differently than the preprocessing solution? * Does anyone have any comments/ideas about my inquiry? I apologize for the lengthy post. Thank you for taking the time to read it. I hope everyone has a blessed day! This e-mail transmission, and any documents, files or previous e-mail messages attached to it, may contain confidential information. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of any of the information contained in or attached to this message is strictly prohibited. If you have received this transmission in error, please immediately notify Jonathan Jennings by reply email or by telephone 1-855-705-1776, and destroy the original transmission and its attachments without reading them or saving them to any media storage device. ------------------------------ Message: 2 Date: Tue, 16 Feb 2016 18:12:27 +0000 (UTC) From: Rene J Buesa <rjbu...@yahoo.com> To: "Vickroy, James" <jvick...@springfieldclinic.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Nuclear Bubbling Message-ID: <656599459.4038172.1455646347478.javamail.ya...@mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 If I remember correctly, this issue has been discussed previously.The general consensus as to the cause of nuclear "bubbling" (in reality a lack of staining in the nuclear area) has been attributed to an incomplete section drying.After the section has be "fished" from the water bath, if the slide is not set to drain the underneath water before drying, the nuclear components are dissolved hence when the section is stained, there is nothing to stain ? "nuclear bubbling".I think this has been previously stated so I really do not understand posting this same question again.I do not think that posting again the question a different answer is going to be received.ren? On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" <histonet@lists.utsouthwestern.edu> wrote: Struggling to find an answer.? We do a lot of GI biopsies in our lab.? Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense.? Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times.? I do not find that the problem is fixation.? In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend).? There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining.? I really thought that this might be the issue however I'm not sure at this point.? Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without.? I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue.? Here are my questions: 1.? ? ? ? Could it be something that is happening with the tissue before it gets to the lab?? Usually a delay if fixation? causes other artifacts but not bubbling.? Could it be heat from the GI procedure? 2.? ? ? We do use blue sponges for our biopsies.? I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3.? ? ? What about the heat stage in our Prisma stainer? I am really getting frustrated.? Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling".? Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue.? I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois? 62703 Office:? 217-528-7541, Ext. 15121 Email:? jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com> This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 16 Feb 2016 18:34:46 +0000 From: Brent Adams <bad...@acadianagastro.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] (CLRW) Clinical Laboratory Reagent Water Message-ID: <cy1pr0301mb164280a887a8f988b931e45bb1...@cy1pr0301mb1642.namprd03.prod.outlook.com> Content-Type: text/plain; charset="Windows-1252" Deanne, I purchase 5 liter cubes from Mercedes Medical. They can provide test results on each batch of water and fax to you. I did have a CLIA inspector advise to document daily on the water as is written on the cube for clarity of water, no sediments or signs of contamination. Think 5 Liters is about $12.50 and I use a little less than two a month. Brent Adams ? BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 ________________________________________ From: histonet-requ...@lists.utsouthwestern.edu <histonet-requ...@lists.utsouthwestern.edu> Sent: Tuesday, February 16, 2016 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 147, Issue 15 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. (CLRW) - Clinical Laboratory Reagent Water (Knutson, Deanne) 2. Tissue cassette baskets for VIP 6 (Vickroy, James) 3. MICROTOME KNIFE SHARPENING (Hannen, Valerie) 4. Re: (CLRW) - Clinical Laboratory Reagent Water (Morken, Timothy) 5. Nuclear Bubbling (Vickroy, James) 6. Complementary webinar on post-processing scientific images, Feb 24 @ 1PM EST (J. Sedgewick) ---------------------------------------------------------------------- Message: 1 Date: Mon, 15 Feb 2016 12:14:06 -0600 From: "Knutson, Deanne" <dknut...@primecare.org> To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] (CLRW) - Clinical Laboratory Reagent Water Message-ID: <1e0e2b14c709174b8ac2be0ae7f76833a4d5970...@exchange2k7.staprimecare.org> Content-Type: text/plain; charset="us-ascii" Fellow Histonetters, Our medical center will no longer be providing us with water for our laboratory procedures. I was wondering where other labs who need to purchase Clinical Laboratory Reagent Water are doing so? Thank you for your help! Deanne Knutson Supervisor Anatomic Pathology ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ------------------------------ Message: 2 Date: Mon, 15 Feb 2016 19:12:06 +0000 From: "Vickroy, James" <jvick...@springfieldclinic.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Tissue cassette baskets for VIP 6 Message-ID: <9b1a1501a800064397369bd8072e6bca06502...@e2k10db.springfieldclinic.com> Content-Type: text/plain; charset="us-ascii" Anybody have an idea where we can get a used cassette basket that will fit in the VIP 6? The cost of a new one is pretty high? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com> This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ------------------------------ Message: 3 Date: Tue, 16 Feb 2016 10:28:59 -0500 From: "Hannen, Valerie" <valerie.han...@parrishmed.com> To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Subject: [Histonet] MICROTOME KNIFE SHARPENING Message-ID: <450B7A81EDA0C54E97C53D60F00776C323546750BC@isexstore03> Content-Type: text/plain; charset="us-ascii" Hi all... Hoping you might be answer a couple of questions that I have. 1) Does anyone use a microtome knife sharpening kit made by Pathco? If so, 2) How well does it sharpen the blades? 3) Is there any issue on attaching the abrasive sheets to the honing plate? 4) Do the abrasive sheets tend to "move" during the sharpening process, that would cause the blade not be on them but on the plate instead? I currently am using coarse and fine abrasive liquids along with the honing plate... but the coarse, fine and honing liquids are becoming quite expensive and hard to come by. Any and all replies are welcomed. Thank you in advance, Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.han...@parrishmed.com<mailto:valerie.han...@parrishmed.com> www.parrishmed.com ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== ------------------------------ Message: 4 Date: Tue, 16 Feb 2016 16:05:12 +0000 From: "Morken, Timothy" <timothy.mor...@ucsf.edu> To: "Knutson, Deanne" <dknut...@primecare.org> Cc: Histonet <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] (CLRW) - Clinical Laboratory Reagent Water Message-ID: <761e2b5697f795489c8710bcc72141ff6fd17...@ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" Deanne, If you just need it for a few critical reagents You can get type 1 water by the pint or the gallon from Fisher (NERL Type 1 water). It's probably overkill for most histology procedures, but convenient if you need it. Note that the expiry clock of 30 days starts when you open the bottle. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Knutson, Deanne via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, February 15, 2016 10:14 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] (CLRW) - Clinical Laboratory Reagent Water Fellow Histonetters, Our medical center will no longer be providing us with water for our laboratory procedures. I was wondering where other labs who need to purchase Clinical Laboratory Reagent Water are doing so? Thank you for your help! Deanne Knutson Supervisor Anatomic Pathology ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 16 Feb 2016 17:10:40 +0000 From: "Vickroy, James" <jvick...@springfieldclinic.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Nuclear Bubbling Message-ID: <9b1a1501a800064397369bd8072e6bca06503...@e2k10db.springfieldclinic.com> Content-Type: text/plain; charset="us-ascii" Struggling to find an answer. We do a lot of GI biopsies in our lab. Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense. Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times. I do not find that the problem is fixation. In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend). There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining. I really thought that this might be the issue however I'm not sure at this point. Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without. I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue. Here are my questions: 1. Could it be something that is happening with the tissue before it gets to the lab? Usually a delay if fixation causes other artifacts but not bubbling. Could it be heat from the GI procedure? 2. We do use blue sponges for our biopsies. I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3. What about the heat stage in our Prisma stainer? I am really getting frustrated. Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling". Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue. I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com> This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ------------------------------ Message: 6 Date: Tue, 16 Feb 2016 11:31:27 -0600 From: "J. Sedgewick" <jerrysedgew...@gmail.com> To: <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Complementary webinar on post-processing scientific images, Feb 24 @ 1PM EST Message-ID: <1557B579CB2A478B884FD20B9E5064D6@sedge> Content-Type: text/plain; format=flowed; charset="UTF-8"; reply-type=original Hello All, Do you post-process your scientific images? Typical tools for common adjustments include Photoshop and ImageJ, but they aren?t the best answer. You are invited to attend a complementary webinar on Feb 24 at 1:00PM EST ? ?Best Practices for Post-Processing of Scientific Images? Reserve your webinar seat now at https://attendee.gotowebinar.com/register/1379379490730827010 I'll be giving this webinar. Also, a similar presentation will be given for the histology WOW 2016 with Dr. Michael Linden at the University of Minnesota on March 25. Best, Jerry Sedgewick ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 147, Issue 15 ***************************************** PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. ------------------------------ Message: 4 Date: Tue, 16 Feb 2016 11:33:47 -0800 From: Jennifer MacDonald <jmacdon...@mtsac.edu> To: Rene J Buesa <rjbu...@yahoo.com> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "Vickroy, James" <jvick...@springfieldclinic.com> Subject: Re: [Histonet] Nuclear Bubbling Message-ID: <of6ccd2b49.a88fcd99-on88257f5b.006b4e2b-88257f5b.006b7...@mtsac.edu> Content-Type: text/plain; charset="GB2312" There is no need to be rude. He has tried the drying option and is still having nuclear bubbling. He is exploring other possible issues. You would see this if you read the email in its entirety. From: Rene J Buesa via Histonet <histonet@lists.utsouthwestern.edu> To: "Vickroy, James" <jvick...@springfieldclinic.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Date: 02/16/2016 10:13 AM Subject: Re: [Histonet] Nuclear Bubbling If I remember correctly, this issue has been discussed previously.The general consensus as to the cause of nuclear "bubbling" (in reality a lack of staining in the nuclear area) has been attributed to an incomplete section drying.After the section has be "fished" from the water bath, if the slide is not set to drain the underneath water before drying, the nuclear components are dissolved hence when the section is stained, there is nothing to stain ? "nuclear bubbling".I think this has been previously stated so I really do not understand posting this same question again.I do not think that posting again the question a different answer is going to be received.ren? On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" <histonet@lists.utsouthwestern.edu> wrote: Struggling to find an answer. We do a lot of GI biopsies in our lab. Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense. Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times. I do not find that the problem is fixation. In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend). There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining. I really thought that this might be the issue however I'm not sure at this point. Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without. I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue. Here are my questions: 1. Could it be something that is happening with the tissue before it gets to the lab? Usually a delay if fixation causes other artifacts but not bubbling. Could it be heat from the GI procedure? 2. We do use blue sponges for our biopsies. I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3. What about the heat stage in our Prisma stainer? I am really getting frustrated. Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling". Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue. I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.com< mailto:jvick...@springfieldclinic.com> This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 16 Feb 2016 14:44:52 -0500 From: "Manfre, Philip" <philip_man...@merck.com> To: Rene J Buesa <rjbu...@yahoo.com>, "Vickroy, James" <jvick...@springfieldclinic.com> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Nuclear Bubbling Message-ID: <558a4571351d0c42bd923f403f4198c40109fe02d...@usctmxp51014.merck.com> Content-Type: text/plain; charset="utf-8" Sort of a rude response to someone looking for help. -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, February 16, 2016 1:12 PM To: Vickroy, James; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Bubbling If I remember correctly, this issue has been discussed previously.The general consensus as to the cause of nuclear "bubbling" (in reality a lack of staining in the nuclear area) has been attributed to an incomplete section drying.After the section has be "fished" from the water bath, if the slide is not set to drain the underneath water before drying, the nuclear components are dissolved hence when the section is stained, there is nothing to stain ? "nuclear bubbling".I think this has been previously stated so I really do not understand posting this same question again.I do not think that posting again the question a different answer is going to be received.ren? On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" <histonet@lists.utsouthwestern.edu> wrote: Struggling to find an answer.? We do a lot of GI biopsies in our lab.? Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense.? Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times.? I do not find that the problem is fixation.? In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend).? There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining.? I really thought that this might be the issue however I'm not sure at this point.? Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without.? I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue.? Here are my questions: 1.? ? ? ? Could it be something that is happening with the tissue before it gets to the lab?? Usually a delay if fixation? causes other artifacts but not bubbling.? Could it be heat from the GI procedure? 2.? ? ? We do use blue sponges for our biopsies.? I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3.? ? ? What about the heat stage in our Prisma stainer? I am really getting frustrated.? Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling".? Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue.? I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois? 62703 Office:? 217-528-7541, Ext. 15121 Email:? jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com> This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 6 Date: Tue, 16 Feb 2016 22:56:28 +0300 From: Jamal Rowaihi <j.rowa...@alborglaboratories.com> To: "Manfre, Philip" <philip_man...@merck.com>, Rene J Buesa <rjbu...@yahoo.com>, "Vickroy, James" <jvick...@springfieldclinic.com> Cc: ???? ??????? <j.rowa...@alborglaboratories.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Nuclear Bubbling Message-ID: <pwsp90twv5eo949blvukh4jo.1455652588...@email.android.com> Content-Type: text/plain; charset=utf-8 Great, I agree? Regards Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories?Sent from my cell phone-------- Original message --------From: "Manfre, Philip via Histonet" <histonet@lists.utsouthwestern.edu> Date: 2/16/2016 10:44 PM (GMT+03:00) To: Rene J Buesa <rjbu...@yahoo.com>, "Vickroy, James" <jvick...@springfieldclinic.com> Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Bubbling Sort of a rude response to someone looking for help. -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, February 16, 2016 1:12 PM To: Vickroy, James; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Bubbling If I remember correctly, this issue has been discussed previously.The general consensus as to the cause of nuclear "bubbling" (in reality a lack of staining in the nuclear area) has been attributed to an incomplete section drying.After the section has be "fished" from the water bath, if the slide is not set to drain the underneath water before drying, the nuclear components are dissolved hence when the section is stained, there is nothing to stain ? "nuclear bubbling".I think this has been previously stated so I really do not understand posting this same question again.I do not think that posting again the question a different answer is going to be received.ren? ??? On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" <histonet@lists.utsouthwestern.edu> wrote: Struggling to find an answer.? We do a lot of GI biopsies in our lab.? Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense.? Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times.? I do not find that the problem is fixation.? In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend).? There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining.? I really thought that this might be the issue however I'm not sure at this point.? Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without.? I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue.? Here are my questions: 1.? ? ? ? Could it be something that is happening with the tissue before it gets to the lab?? Usually a delay if fixation? causes other artifacts but not bubbling.? Could it be heat from the GI procedure? 2.? ? ? We do use blue sponges for our biopsies.? I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3.? ? ? What about the heat stage in our Prisma stainer? I am really getting frustrated.? Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling".? Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue.? I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois? 62703 Office:? 217-528-7541, Ext. 15121 Email:? jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com> This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice:? This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 16 Feb 2016 21:53:00 +0000 From: "Vickroy, James" <jvick...@springfieldclinic.com> To: 'Jamal Rowaihi' <j.rowa...@alborglaboratories.com>, "Manfre, Philip" <philip_man...@merck.com>, Rene J Buesa <rjbu...@yahoo.com> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Nuclear Bubbling Message-ID: <9b1a1501a800064397369bd8072e6bca06504...@e2k10db.springfieldclinic.com> Content-Type: text/plain; charset="utf-8" For the record please note that I have over thirty-six years experience working in a Histology lab. I have been a supervisor or manager of a hospital and clinic histology department for at least 25 years. Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com> From: Jamal Rowaihi [mailto:j.rowa...@alborglaboratories.com] Sent: Tuesday, February 16, 2016 1:56 PM To: Manfre, Philip; Rene J Buesa; Vickroy, James Cc: ???? ???????; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Bubbling Great, I agree Regards Jamal Rowaihi Anatomic Pathology Supervisor Al Borg Medical Laboratories Sent from my cell phone -------- Original message -------- From: "Manfre, Philip via Histonet" <histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> Date: 2/16/2016 10:44 PM (GMT+03:00) To: Rene J Buesa <rjbu...@yahoo.com<mailto:rjbu...@yahoo.com>>, "Vickroy, James" <jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com>> Cc: histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Nuclear Bubbling Sort of a rude response to someone looking for help. -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, February 16, 2016 1:12 PM To: Vickroy, James; histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Nuclear Bubbling If I remember correctly, this issue has been discussed previously.The general consensus as to the cause of nuclear "bubbling" (in reality a lack of staining in the nuclear area) has been attributed to an incomplete section drying.After the section has be "fished" from the water bath, if the slide is not set to drain the underneath water before drying, the nuclear components are dissolved hence when the section is stained, there is nothing to stain ? "nuclear bubbling".I think this has been previously stated so I really do not understand posting this same question again.I do not think that posting again the question a different answer is going to be received.ren? On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" <histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> wrote: Struggling to find an answer. We do a lot of GI biopsies in our lab. Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense. Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times. I do not find that the problem is fixation. In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend). There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining. I really thought that this might be the issue however I'm not sure at this point. Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without. I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue. Here are my questions: 1. Could it be something that is happening with the tissue before it gets to the lab? Usually a delay if fixation causes other artifacts but not bubbling. Could it be heat from the GI procedure? 2. We do use blue sponges for our biopsies. I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3. What about the heat stage in our Prisma stainer? I am really getting frustrated. Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling". Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue. I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com%3cmailto:jvick...@springfieldclinic.com>> This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 16 Feb 2016 21:56:49 +0000 From: "Carlos Defeo" <late...@adinet.com.uy> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] nuclear bubbling Message-ID: <em1942d68c-0573-4d27-a81c-0bed93cc738c@calipc> Content-Type: text/plain; format=flowed; charset=utf-8 Dear James: Two chained events take place on your issue: 1-sections not entirely drained,water remains even minimally under sections; 2- you put these sections in the oven, the heat literally "explodes" the nuclear bubbles and creates a hole with no cromatin to stain. My kind regards, Carlos Defeo Histotechnologist ------------------------------ Message: 9 Date: Tue, 16 Feb 2016 16:18:03 -0600 From: Katie Sands <derm.katiesa...@gmail.com> To: "Vickroy, James" <jvick...@springfieldclinic.com> Cc: Jamal Rowaihi <j.rowa...@alborglaboratories.com>, "Manfre, Philip" <philip_man...@merck.com>, Rene J Buesa <rjbu...@yahoo.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Nuclear Bubbling Message-ID: <caorf-nhjeszx1sc1bviwza84o0fqjy5vjgtz33fhfvghzei...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 I don't have your answer Jim, yet I can vouch for your experience as a supervisor since you were my boss for about two years. Hopefully you can get it resolved because it can be very frustrating as the tech. On Tuesday, February 16, 2016, Vickroy, James via Histonet < histonet@lists.utsouthwestern.edu> wrote: > For the record please note that I have over thirty-six years experience > working in a Histology lab. I have been a supervisor or manager of a > hospital and clinic histology department for at least 25 years. > > Jim Vickroy > Histology Manager > Springfield Clinic, Main Campus, East Building > 1025 South 6th Street > Springfield, Illinois 62703 > Office: 217-528-7541, Ext. 15121 > Email: jvick...@springfieldclinic.com<mailto: > jvick...@springfieldclinic.com <javascript:;>> > > > From: Jamal Rowaihi [mailto:j.rowa...@alborglaboratories.com > <javascript:;>] > Sent: Tuesday, February 16, 2016 1:56 PM > To: Manfre, Philip; Rene J Buesa; Vickroy, James > Cc: ???? ???????; histonet@lists.utsouthwestern.edu <javascript:;> > Subject: Re: [Histonet] Nuclear Bubbling > > Great, I agree > > > > Regards > > Jamal Rowaihi > Anatomic Pathology Supervisor > Al Borg Medical Laboratories > Sent from my cell phone > -------- Original message -------- > From: "Manfre, Philip via Histonet" <histonet@lists.utsouthwestern.edu > <javascript:;><mailto:histonet@lists.utsouthwestern.edu <javascript:;>>> > Date: 2/16/2016 10:44 PM (GMT+03:00) > To: Rene J Buesa <rjbu...@yahoo.com <javascript:;><mailto: > rjbu...@yahoo.com <javascript:;>>>, "Vickroy, James" > <jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com > <javascript:;>>> > Cc: histonet@lists.utsouthwestern.edu <javascript:;><mailto: > histonet@lists.utsouthwestern.edu <javascript:;>> > Subject: Re: [Histonet] Nuclear Bubbling > > Sort of a rude response to someone looking for help. > > -----Original Message----- > From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu > <javascript:;>] > Sent: Tuesday, February 16, 2016 1:12 PM > To: Vickroy, James; histonet@lists.utsouthwestern.edu <javascript:;> > <mailto:histonet@lists.utsouthwestern.edu <javascript:;>> > Subject: Re: [Histonet] Nuclear Bubbling > > If I remember correctly, this issue has been discussed previously.The > general consensus as to the cause of nuclear "bubbling" (in reality a lack > of staining in the nuclear area) has been attributed to an incomplete > section drying.After the section has be "fished" from the water bath, if > the slide is not set to drain the underneath water before drying, the > nuclear components are dissolved hence when the section is stained, there > is nothing to stain ? "nuclear bubbling".I think this has been previously > stated so I really do not understand posting this same question again.I do > not think that posting again the question a different answer is going to be > received.ren? > > On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" < > histonet@lists.utsouthwestern.edu <javascript:;><mailto: > histonet@lists.utsouthwestern.edu <javascript:;>>> wrote: > > > > Struggling to find an answer. We do a lot of GI biopsies in our lab. > Sometimes they look wonderful without any nuclear bubbling, other times the > bubbling is pretty intense. Since nuclear bubbling is often attributed to > incomplete fixation we of course have investigated the fixation times. I > do not find that the problem is fixation. In fact some of the biopsies end > up fixing for 48 hrs before processing. (weekend). There was a suggestion > last week or so that there might be water trapped under the slides after > cutting and before staining. I really thought that this might be the issue > however I'm not sure at this point. Extra drying seems to help but > sometimes slides side by side are so variable, one with bubbles and one > without. I also don't believe the problem is in the processing schedule > since the problem has shown up on both a rapid and a normal schedule. > (therefore longer dehydration, clearing, etc.) > > I am wondering if anyone else has worked with this issue. Here are my > questions: > > > 1. Could it be something that is happening with the tissue before > it gets to the lab? Usually a delay if fixation causes other artifacts > but not bubbling. Could it be heat from the GI procedure? > > 2. We do use blue sponges for our biopsies. I know some say get rid > of the sponges but has anyone seen this problem caused by usage of sponges? > > 3. What about the heat stage in our Prisma stainer? > > > I am really getting frustrated. Pathologists never complain however I > would rather all of the tissue did not have the "nuclear bubbling". Again > we only do biopsies so I really don't think the standard old " not enough > time in formalin" is the issue. I have even wondered about variables such > as we use recycled formalin, recycled Clearite III. > > Any suggestions? > > Jim > > > > Jim Vickroy > Histology Manager > Springfield Clinic, Main Campus, East Building > 1025 South 6th Street > Springfield, Illinois 62703 > Office: 217-528-7541, Ext. 15121 > Email: jvick...@springfieldclinic.com<mailto: > jvick...@springfieldclinic.com <javascript:;><mailto: > jvick...@springfieldclinic.com <javascript:;> > %3cmailto:jvick...@springfieldclinic.com>> > > > > This electronic message contains information from Springfield Clinic, LLP > that may be confidential, privileged, and/or sensitive. This information is > intended for the use of the individual(s) or entity(ies) named above. If > you are not the intended recipient, be aware that disclosure, copying, > distribution, or action taken on the contents of this information is > strictly prohibited. If you have received this electronic message in error, > please notify the sender immediately, by electronic mail, so that > arrangements may be made for the retrieval of this electronic message. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu <javascript:;><mailto: > Histonet@lists.utsouthwestern.edu <javascript:;>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu <javascript:;><mailto: > Histonet@lists.utsouthwestern.edu <javascript:;>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, > New Jersey, USA 07033), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu <javascript:;><mailto: > Histonet@lists.utsouthwestern.edu <javascript:;>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu <javascript:;> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Katie L. Sands Histology Technician/Lab Manager Advanced Dermatology of Southeast Missouri, PC 2116 Megan Drive Suite 102 Cape Girardeau, MO 63701 derm.katiesa...@gmail.com Work: (573) 335-7546 Cell: (309) 840-3799 www.dermsemo.com ------------------------------ Message: 10 Date: Wed, 17 Feb 2016 16:31:51 +0000 From: Carlos Genty <cge...@criticalxsolutions.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Sequenza Immunostaining Racks Message-ID: <bn3pr0101mb1089d98081a162c8cad05163ae...@bn3pr0101mb1089.prod.exchangelabs.com> Content-Type: text/plain; charset="iso-8859-1" Good Day Everyone, I am looking to obtain used Thermo Sequenza Immunostaining Racks (about 10 or so). Does anyone have any that they would be willing to part with? Please feel free to contact me directly. Thanks in advance! Carlos Carlos Genty Critical X Solutions, LLC cge...@criticalxsolutions.com (310) 357-1993 ------------------------------ Message: 11 Date: Wed, 17 Feb 2016 10:03:45 -0700 From: Jb <craiga...@gmail.com> To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Error Prevention: Message-ID: <9ed89d91-cb8e-4014-b24a-6c9131441...@gmail.com> Content-Type: text/plain; charset=us-ascii Does anyone have a policy that they are willing to share regarding tech errors, error prevention, etc? How do you document, retrain, write up, and terminate employees for errors? What is considered severe problems for termination, etc. I know it is up to the manager, I want to know what other lab standards are to help create a procedure. Thank you, Sent from my iPhone ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 147, Issue 16 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
