Is this a good fixative for fetal brain? Anybody have any suggestions as to protocols? Do any of the readers here do fetal (human) brain on a regular basis?
----- Original Message ----- From: "Bob Richmond via Histonet" <histonet@lists.utsouthwestern.edu> To: "Histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Sent: Tuesday, February 23, 2016 3:22:50 PM Subject: Re: [Histonet] Davidson's fixative and IHC question Several queries about the possible effect of Davidson's fixative on immunohistochemistry. I have a lot of experience with Davidson's fixative, but none with its effect on IHC. Davidson's fixative is easily prepared: 3 parts tap water 3 parts ethanol or reagent alcohol 2 parts 37% formalin (not neutral buffered formalin) 1 part glacial acetic acid It is quite destructive to H & E staining if fixation is at all prolonged. Tissue should be taken out of it in not more much more than 24 hours. The history of Davidson's fixative is somewhat obscure - I have a good bit of information on it. John Kiernan has said he'll work on ascertaining more about its history if he ever has the time. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
