Cool trick Jeffrey! On Tue, May 10, 2016 at 12:14 PM, Maxim Peshkov via Histonet < histonet@lists.utsouthwestern.edu> wrote:
> Karen, > The bleaching reagents will not compatible before IHC anyway. > Some tips: > 1. Do IHC-test as usual and counterstain nuclei with methylene blue > Loeffler at 3-5 sec, only for > profile of chromatine. The melanin will stain at green color. > 2. Use any red color chromogen for IHC. It will be some contrast with > brown melanine > 3. Cut sections at 3 microns. Do all steps of IHC as usual, include DAB. > All next steps do very gently: > Before nuclear counterstain do bleaching procedure with 0.01% KMnO4 + 0.5% > H2SO4 at 10 mins; > gently rinse in DW 1 min; > 0.5% oxalic acid 5-10 sec or up to bleaching; > gently rinse in DW 1 min; > counterstain nuclei and all other steps with regular manner of your lab. > This solutions will not bleach DAB. > Sections will not detach from slides. > > Hope this help. > -- > Maxim Peshkov > Russia > Taganrog. mailto:maxim...@mail.ru > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
