You are right. Bleaching is a "rough" procedure for the "survival" of sections
and if on top of that you left the section overnight in DiH2O that is a recipe
for disaster, as the one you experienced. Try to do the whole procedure during
the same day.Additionally it seems to me that 6h in potassium permanganate is
too much, you should check the condition of the sections every hour trying to
minimize KMnO4 action the least time possible.René
On Tuesday, May 10, 2016 12:10 PM, "Heckford, Karen - SMMC-SF via Histonet"
<histonet@lists.utsouthwestern.edu> wrote:
Good Morning,
I need some help. Yesterday I bleached some heavily pigmented tissue. I have
to run some IHC's on them. I bought the bleaching kit from American Master
Tech. I had to put the Permanganate for about 6 hours to get the melanin and
then a couple of minutes in Oxalic Acid. I had to let them set overnight
because I could not get another IHC run in that day. It looks like the tissue
fell off during decloaking. I used Apex slides. I rarely ever have to
bleach anything here. So I am not sure if I did this correctly. I am thinking
I need to bleach and do the run in the same day and not let them set over night
in DiH20.
Thanks,
Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org
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