Hello Histonetters, I'm having an issue with some recent samples and was wondering if anyone else has experienced the same thing...I stained some samples with a previously optimized protocol for anti-human Granzyme B on the Ventana Benchmark and about 1/3 of my samples did not stain at all with either GranzB or Hematoxylin. My controls and samples that did stain positive for GranzB look fine and the machine has been recently serviced and working well with other runs. I'd really appreciate your input if you have any advice or suggestions on what could have gone wrong.
Thanks, Anna _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
