Anna wrote:
I'm curious as to why we heat the Auramine Rhodamine when we are staining 
tissue sections but our Micro lab stains at room temp.  It is because "it's 
always been done that way"?
Thanks group,
Angie

Hi Anna,

If your dispensers are working properly, I would bet it's your slides. There 
are known issues with overly hydrophobic slides causing issues on the Ventana 
platform. They recommend you use Plus (+) slides manufactured from Erie 
Scientific. There may be others I am not aware of, but we use these slides for 
all IHC sectioned here and have had no issues on our Ventanas.

Good luck!

Teri Johnson, HT(ASCP)QIHC
Manager Clinical Trial Testing
Genoptix, Inc.
SAN5, Rm. 2005
760.516.5954 (office)
760.516.6201 (fax)




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