Dear Histoneters!Good DayMay I request you guys to share protocol of frozen
tissues (mouse liver) for immunoflorescence assay. Lots of protocol are
available online but i will highly appreciate the one that could lead me from
tissue preparation towards staining procedure as I am really blank about frozen
tissues and slides dealing. So I will be on standby for your response. Thanks
in advance.
Sincerely Nasir Abbas
---PhD FellowGuangzhou Institutes of Biomedicine and HealthChinese Academy of
Sciences190 Kai Yuan Avenue Science Park Guangzhou, 510530, Guangdong
P.R.ChinaCell: +86-1312 8296 614
On Wednesday, 28 September 2016, 1:12,
"histonet-requ...@lists.utsouthwestern.edu"
<histonet-requ...@lists.utsouthwestern.edu> wrote:
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Today's Topics:
1. GMS (Martin, Gary)
2. Re: Unprocessing tissue (Caroline Miller)
3. Water for H&E Stainers? (P Sicurello)
4. Re: Water for H&E Stainers? (Elizabeth Chlipala)
----------------------------------------------------------------------
Message: 1
Date: Mon, 26 Sep 2016 16:30:49 -0700
From: "Martin, Gary" <gmar...@marshallmedical.org>
To: <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] GMS
Message-ID:
<6ED9D4252F278841A0593D3D788AF24C366E5E51@mailsvr.MARSHMED.local>
Content-Type: text/plain; charset="us-ascii"
I am having difficulties with a stain I've done many times ...GMS. The
light green is only staining the outer edges of the specimen. Any Ideas?
Thanks
Gary
------------------------------
Message: 2
Date: Mon, 26 Sep 2016 16:49:14 -0700
From: Caroline Miller <mi...@3scan.com>
To: "Armstrong, Karoleigh T" <karoleigh.armstr...@armc.net>
Cc: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Unprocessing tissue
Message-ID:
<CAFTHRQMn5=hqzoyf-frj-mf2agzeup_gzwyjxnhgux90hmk...@mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Not crazy at all, this is the kind of thing I was thinking someone would
have out there :)
I have used downey on hard tissue block faces before now too!
thanks so much for all your advice histonet :)
mills
On Fri, Sep 23, 2016 at 12:44 PM, Armstrong, Karoleigh T via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Hi Caroline,
>
> This may sound a little crazy,
>
> but I was working on some mummified tissue back in the 80's, (Mommy is
> old), and to deal with the dried out tissue we would give them,
>
> 1. A quick .5 percent Sodium Hydroxide soak, 5minutes
>
> 2. Wash is running water, 1 minute
>
> 3. Soak in a 3 parts water 1 part Downey, yes Downey. 5 minutes
>
> 4. Step up to a 1/2 water, 1/2 Downey soak for 5- 10 minutes.
>
> 5. Wash with running water 2-3 minutes.
>
> Proscess your tissue as you usually would.
>
> They came out pretty darn good.
>
> KK Armstrong H.T. (ASCP)
>
>
> --------------------------------------------------------------------------
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--
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
------------------------------
Message: 3
Date: Tue, 27 Sep 2016 09:28:25 -0700
From: P Sicurello <pat...@gmail.com>
To: HistoNet <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Water for H&E Stainers?
Message-ID:
<capsjddyceta1fldsufgwzctayssqk6rxdrvtssqjmdwqaju...@mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Good Morning Everyone,
What type of water do you use with your automated H&E stainers? House or
deionized/distilled?
We cannot buy one of the waterless, fancy schmancy H&E stainers at this
time. L
Thank you in advance.
Sincerely,
Paula
Paula Sicurello, HTL (ASCP)CM
Histotechnology Specialist
UC San Diego Health
200 Arbor Drive
San Diego, CA 92103
(P): 619-543-2872
*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material. Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited. If you received this e-mail in error,
please contact the sender and delete the material from any computer.
------------------------------
Message: 4
Date: Tue, 27 Sep 2016 10:54:18 -0600
From: Elizabeth Chlipala <l...@premierlab.com>
To: P Sicurello <pat...@gmail.com>,
"'histonet@lists.utsouthwestern.edu'
(histonet@lists.utsouthwestern.edu)"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Water for H&E Stainers?
Message-ID:
<14E2C6176416974295479C64A11CB9AE02BEEABA5B09@SBS2K8.premierlab.local>
Content-Type: text/plain; charset="us-ascii"
Paula
We have ours hooked up to tap water.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, September 27, 2016 10:28 AM
To: HistoNet
Subject: [Histonet] Water for H&E Stainers?
Good Morning Everyone,
What type of water do you use with your automated H&E stainers? House or
deionized/distilled?
We cannot buy one of the waterless, fancy schmancy H&E stainers at this time.
L Thank you in advance.
Sincerely,
Paula
Paula Sicurello, HTL (ASCP)CM
Histotechnology Specialist
UC San Diego Health
200 Arbor Drive
San Diego, CA 92103
(P): 619-543-2872
*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may contain
confidential and/or privileged material. Any review, retransmission,
dissemination or other use of or taking of any action in reliance upon this
information by persons or entities other than the intended recipient is
prohibited. If you received this e-mail in error, please contact the sender
and delete the material from any computer.
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------------------------------
End of Histonet Digest, Vol 154, Issue 22
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