I can't speak to the the T-cell receptor sites but the only option for getting decent sections at this point is option number two. In the future, (if there applicable) the cut surface of a cryosectioned block can be recovered with OCT, frozen, and stored in an air-tight whirl-pak or ziplock bag in the freezer for a little longer to avoid freezer burn. Cheers, Greg
-- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet