Agreed! Don't believe the 'single use only' on the coverplates - wash and reuse them. I use tube racks to stand them up to dry, then put the back in the original packaging. after a few years some of them might lose their spring and you will need to replace some at that point.
yours, mills On Mon, Feb 13, 2017 at 7:42 AM, Paula Keene Pierce < pa...@excaliburpathology.com> wrote: > https://www.fishersci.com/shop/products/7219950/7219950#?keyword=sequenza > > These are the cover plates. > > I have no financial interest in Thermo Fisher, but have used the system > for years. > > > > > Paula Keene Pierce, BS, HTL(ASCP)HT > President > Excalibur Pathology, Inc. > 5830 N Blue Lake Drive > Norman, OK 73069 > PH 405-759-3953 <(405)%20759-3953> > FAX 405-759-7513 <(405)%20759-7513> > www.excaliburpathology.com > > > ------------------------------ > *From:* Caroline Miller via Histonet <histonet@lists.utsouthwestern.edu> > *To:* Ranna Mehta <ranna0...@gmail.com> > *Cc:* "Histonet@Lists. Edu" <histonet@lists.utsouthwestern.edu> > *Sent:* Monday, February 13, 2017 9:06 AM > *Subject:* Re: [Histonet] hand staining immunos > > This is a great system for semi-automated immuno. I find the results a > little cleaner than performing the technique straight on the slide. No need > to buy the whole set up (it is just a glorified timer)- just the racks and > clips are enough! > > https://www.fishersci.com/shop/products/thermo- > scientific-shandon-sequenza-immunostaining-center-2/p-4010433 > > mills > > On Thu, Feb 9, 2017 at 11:32 AM, Ranna Mehta via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > Hi Jennifer, > > > > How many slides are you planning to stain in routine? Once you will > > understand the basic, it will be a very interesting field. If you like, I > > can help you to walk through process with step by step. send me a message > > through my email.All the best and thanks. > > > > Regards > > Ranna > > > > On Thu, Feb 9, 2017 at 12:00 PM, <histonet-request@lists. > > utsouthwestern.edu> > > wrote: > > > > > Send Histonet mailing list submissions to > > > histonet@lists.utsouthwestern.edu > > > > > > To subscribe or unsubscribe via the World Wide Web, visit > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > or, via email, send a message with subject or body 'help' to > > > histonet-requ...@lists.utsouthwestern.edu > > > > > > You can reach the person managing the list at > > > histonet-ow...@lists.utsouthwestern.edu > > > > > > When replying, please edit your Subject line so it is more specific > > > than "Re: Contents of Histonet digest..." > > > > > > > > > Today's Topics: > > > > > > 1. Looking for a Histology Workshop (Jessica Riggleman) > > > 2. Re: Looking for a Histology Workshop (Jessica Riggleman) > > > 3. hand staining immunos (Jennifer) > > > 4. Re: hand staining immunos (Rene J Buesa) > > > 5. Re: hand staining immunos (Walter Benton) > > > > > > > > > ---------------------------------------------------------------------- > > > > > > Message: 1 > > > Date: Wed, 8 Feb 2017 18:07:24 +0000 > > > From: Jessica Riggleman <jriggle...@globusmedical.com> > > > To: "histonet@lists.utsouthwestern.edu" > > > <histonet@lists.utsouthwestern.edu> > > > Subject: [Histonet] Looking for a Histology Workshop > > > Message-ID: > > > <D0AEE53F1F084340888BEB10C81DE9B295C77FAC@SW10EXM01.gminc.local > > > > > Content-Type: text/plain; charset=UTF-8 > > > > > > > > > Hi Everyone, > > > > > > I am looking for workshop or class for Histology/Histotechnology that > > > lasts anywhere from 1 week to 3 months in the U.S. > > > Preferably in-person and not online. > > > > > > If you have any suggestions or contacts, please let me know. > > > > > > Thank You, > > > Jessica > > > > > > _____________________________________________________________________ > > > > > > Jessica Riggleman | Research Associate > > > > > > Globus Medical, Inc. > > > Valley Forge Business Center > > > 2560 General Armistead Avenue | Audubon, PA 19403 > > > Ph: (610) 930-1800 ext. 2583 <(610)%20930-1800> | Fax: > > > website: http://www.globusmedical.com | email: > > > jriggle...@globusmedical.com<mailto:jriggle...@globusmedical.com> > > > An ISO 13485 Registered Company > > > > > > Confidentiality Note: This email is confidential and intended solely > for > > > the use of the individual to whom it is addressed. If you are not the > > > intended recipient, be advised that you have received this email in > error > > > and that any use, dissemination, forwarding, printing, or copying of > this > > > email is strictly prohibited. If you have received this email in error > > > please contact the sender. Any views or opinions presented are solely > > those > > > of the author and do not necessarily represent those of Globus Medical, > > > Inc. Although this email and any attachments are believed to be free of > > any > > > virus or other defects which might affect any computer or IT system > into > > > which they are received, no responsibility is accepted by Globus > Medical, > > > Inc. for any loss or damage arising in any way from the receipt or use > > > thereof. > > > > > > Disclaimer > > > > > > The information contained in this communication from the sender is > > > confidential. It is intended solely for use by the recipient and others > > > authorized to receive it. If you are not the recipient, you are hereby > > > notified that any disclosure, copying, distribution or taking action in > > > relation of the contents of this information is strictly prohibited and > > may > > > be unlawful. > > > > > > This email has been scanned for viruses and malware, and may have been > > > automatically archived by Mimecast Ltd, an innovator in Software as a > > > Service (SaaS) for business. Providing a safer and more useful place > for > > > your human generated data. Specializing in; Security, archiving and > > > compliance. To find out more visit the Mimecast website. > > > > > > > > > ------------------------------ > > > > > > Message: 2 > > > Date: Wed, 8 Feb 2017 21:33:47 +0000 > > > From: Jessica Riggleman <jriggle...@globusmedical.com> > > > To: Esther C Peters <epete...@gmu.edu> > > > Cc: "histonet@lists.utsouthwestern.edu" > > > <histonet@lists.utsouthwestern.edu> > > > Subject: Re: [Histonet] Looking for a Histology Workshop > > > Message-ID: > > > <D0AEE53F1F084340888BEB10C81DE9B295C780C0@SW10EXM01.gminc.local > > > > > Content-Type: text/plain; charset=UTF-8 > > > > > > Hi Esther, > > > > > > We are looking for basic training to include study design, sectioning > raw > > > specimens for processing (i.e., how large should my specimen be?), > > > processing techniques (fixation, etc.), special stains, and slide > > reading. > > > This is not for HT/HTL exam preparation. > > > > > > We would prefer non-human tissues, as we typically work with large > sheep > > > and rabbit specimens. Only two students. > > > > > > Thank You, > > > Jessica > > > > > > > > > From: Esther C Peters [mailto:epete...@gmu.edu] > > > Sent: Wednesday, February 08, 2017 4:18 PM > > > To: Jessica Riggleman <jriggle...@globusmedical.com> > > > Cc: histonet@lists.utsouthwestern.edu > > > Subject: Re: Looking for a Histology Workshop > > > > > > Is this for human or non-human tissues? How many students? > > > > > > Esther > > > > > > > > > _____________________________________________________________________ > > > > > > Jessica Riggleman | Research Associate > > > > > > Globus Medical, Inc. > > > Valley Forge Business Center > > > 2560 General Armistead Avenue | Audubon, PA 19403 > > > Ph: (610) 930-1800 ext. 2583 <(610)%20930-1800> | Fax: > > > > > > Confidentiality Note: This email is confidential and intended solely > for > > > the use of the individual to whom it is addressed. If you are not the > > > intended recipient, be advised that you have received this email in > error > > > and that any use, dissemination, forwarding, printing, or copying of > this > > > email is strictly prohibited. If you have received this email in error > > > please contact the sender. Any views or opinions presented are solely > > those > > > of the author and do not necessarily represent those of Globus Medical, > > > Inc. Although this email and any attachments are believed to be free of > > any > > > virus or other defects which might affect any computer or IT system > into > > > which they are received, no responsibility is accepted by Globus > Medical, > > > Inc. for any loss or damage arising in any way from the receipt or use > > > thereof. > > > > > > > > > ________________________________________ > > > From: Jessica Riggleman <jriggle...@globusmedical.com> > > > Sent: Wednesday, February 8, 2017 4:12:20 PM > > > To: Esther C Peters > > > Cc: histonet@lists.utsouthwestern.edu > > > Subject: RE: Looking for a Histology Workshop > > > > > > Hi Esther, > > > > > > We are looking for basic training to include study design, sectioning > raw > > > specimens for processing (i.e., how large should my specimen be?), > > > processing techniques (fixation, etc.), special stains, and slide > > reading. > > > This is not for HT/HTL exam preparation. > > > > > > Thank You, > > > Jessica > > > > > > ________________________________________ > > > > > > Jessica Riggleman | Research Associate > > > > > > Globus Medical, Inc. > > > Valley Forge Business Center > > > 2560 General Armistead Avenue | Audubon, PA 19403 > > > Ph: (610) 930-1800 ext. 2583 <(610)%20930-1800> | Fax: > > > An ISO 13485 Registered Company > > > > > > website | map | email > > > > > > > > > > > > > > > > > > Confidentiality Note: This email is confidential and intended solely > for > > > the use of the individual to whom it is addressed. If you are not the > > > intended recipient, be advised that you have received this email in > error > > > and that any use, dissemination, forwarding, printing, or copying of > this > > > email is strictly prohibited. If you have received this email in error > > > please contact the sender. Any views or opinions presented are solely > > those > > > of the author and do not necessarily represent those of Globus Medical, > > > Inc. Although this email and any attachments are believed to be free of > > any > > > virus or other defects which might affect any computer or IT system > into > > > which they are received, no responsibility is accepted by Globus > Medical, > > > Inc. for any loss or damage arising in any way from the receipt or use > > > thereof. > > > From: Esther C Peters [mailto:epete...@gmu.edu] > > > Sent: Wednesday, February 08, 2017 3:53 PM > > > To: Jessica Riggleman <jriggle...@globusmedical.com> > > > Subject: Re: Looking for a Histology Workshop > > > > > > Hi Jessica, > > > > > > It might help everyone to know exactly what you want to cover in the > > > workshop or class. Do you need basic training, preparation for the > HT/HTL > > > exam, slide preparation, special stains, or slide reading? What is your > > > goal? > > > > > > Esther > > > > > > Esther C. Peters, Ph.D. > > > George Mason University > > > Fairfax, VA 22030-4444 > > > ________________________________________ > > > From: Jessica Riggleman via Histonet <histonet@lists. > utsouthwestern.edu> > > > Sent: Wednesday, February 8, 2017 1:07:24 PM > > > To: histonet@lists.utsouthwestern.edu > > > Subject: [Histonet] Looking for a Histology Workshop > > > > > > > > > Hi Everyone, > > > > > > I am looking for workshop or class for Histology/Histotechnology that > > > lasts anywhere from 1 week to 3 months in the U.S. > > > Preferably in-person and not online. > > > > > > If you have any suggestions or contacts, please let me know. > > > > > > Thank You, > > > Jessica > > > > > > _____________________________________________________________________ > > > > > > Jessica Riggleman | Research Associate > > > > > > Globus Medical, Inc. > > > Valley Forge Business Center > > > 2560 General Armistead Avenue | Audubon, PA 19403 > > > Ph: (610) 930-1800 ext. 2583 <(610)%20930-1800> | Fax: > > > website: http://www.globusmedical.com | email: > > > jriggle...@globusmedical.com<mailto:jriggle...@globusmedical.com> > > > An ISO 13485 Registered Company > > > > > > Confidentiality Note: This email is confidential and intended solely > for > > > the use of the individual to whom it is addressed. If you are not the > > > intended recipient, be advised that you have received this email in > error > > > and that any use, dissemination, forwarding, printing, or copying of > this > > > email is strictly prohibited. If you have received this email in error > > > please contact the sender. Any views or opinions presented are solely > > those > > > of the author and do not necessarily represent those of Globus Medical, > > > Inc. Although this email and any attachments are believed to be free of > > any > > > virus or other defects which might affect any computer or IT system > into > > > which they are received, no responsibility is accepted by Globus > Medical, > > > Inc. for any loss or damage arising in any way from the receipt or use > > > thereof. > > > > > > Disclaimer > > > > > > The information contained in this communication from the sender is > > > confidential. It is intended solely for use by the recipient and others > > > authorized to receive it. If you are not the recipient, you are hereby > > > notified that any disclosure, copying, distribution or taking action in > > > relation of the contents of this information is strictly prohibited and > > may > > > be unlawful. > > > > > > This email has been scanned for viruses and malware, and may have been > > > automatically archived by Mimecast Ltd, an innovator in Software as a > > > Service (SaaS) for business. Providing a safer and more useful place > for > > > your human generated data. Specializing in; Security, archiving and > > > compliance. To find out more visit the Mimecast website. > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > Disclaimer > > > > > > The information contained in this communication from the sender is > > > confidential. It is intended solely for use by the recipient and others > > > authorized to receive it. If you are not the recipient, you are hereby > > > notified that any disclosure, copying, distribution or taking action in > > > relation of the contents of this information is strictly prohibited and > > may > > > be unlawful. > > > > > > This email has been scanned for viruses and malware, and may have been > > > automatically archived by Mimecast Ltd, an innovator in Software as a > > > Service (SaaS) for business. Providing a safer and more useful place > for > > > your human generated data. Specializing in; Security, archiving and > > > compliance. To find out more visit the Mimecast website. > > > > > > ------------------------------ > > > > > > Message: 3 > > > Date: Wed, 8 Feb 2017 16:16:10 -0800 > > > From: "Jennifer" <histot...@dermspec.com> > > > To: <histonet@lists.utsouthwestern.edu> > > > Subject: [Histonet] hand staining immunos > > > Message-ID: <000001d28269$b81e82f0$285b88d0$@com> > > > Content-Type: text/plain; charset="us-ascii" > > > > > > Hello, > > > > > > > > > > > > My dermatopathologist wants me to start hand staining immunos for > MITF. > > I > > > would really like some feedback about everyone's experiences/thoughts > > with > > > manually hand staining immunos and especially with MITF. Pros and > cons? I > > > am > > > concerned about the amount of time this will take for a tech to babysit > > the > > > stain and also about the consistency of the quality in the end result. > > > Your > > > opinions are much appreciated. > > > > > > > > > > > > Thank you! > > > > > > > > > > > > Jennifer > > > > > > > > > > > > ------------------------------ > > > > > > Message: 4 > > > Date: Thu, 9 Feb 2017 15:51:22 +0000 (UTC) > > > From: Rene J Buesa <rjbu...@yahoo.com> > > > To: Jennifer <histot...@dermspec.com>, > > > "histonet@lists.utsouthwestern.edu" > > > <histonet@lists.utsouthwestern.edu> > > > Subject: Re: [Histonet] hand staining immunos > > > Message-ID: <267846266.1416224.1486655482...@mail.yahoo.com> > > > Content-Type: text/plain; charset=UTF-8 > > > > > > You do not have to "babysit" the procedure for it has well > defined/timed > > > steps. You just need a timer and check the slides when required. As to > > > procedural "dos & donts" try to get a copy of the DAKO IHC manual.Ren? > > > > > > On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet < > > > histonet@lists.utsouthwestern.edu> wrote: > > > > > > > > > Hello, > > > > > > > > > > > > My dermatopathologist wants me to start hand staining immunos? for > MITF. > > I > > > would really like some feedback about everyone's experiences/thoughts > > with > > > manually hand staining immunos and especially with MITF. Pros and > cons? I > > > am > > > concerned about the amount of time this will take for a tech to babysit > > the > > > stain and also about the consistency of the quality in the end result.? > > > Your > > > opinions are much appreciated. > > > > > > > > > > > > Thank you! > > > > > > > > > > > > Jennifer > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > > > > ------------------------------ > > > > > > Message: 5 > > > Date: Thu, 9 Feb 2017 16:19:00 +0000 > > > From: Walter Benton <wben...@cua.md> > > > To: Rene J Buesa <rjbu...@yahoo.com>, Jennifer > > > <histot...@dermspec.com> > > > Cc: "histonet@lists.utsouthwestern.edu" > > > <histonet@lists.utsouthwestern.edu> > > > Subject: Re: [Histonet] hand staining immunos > > > Message-ID: <0952dbba122f4678aa83759b2c98d...@mail01.gcu-MD.local> > > > Content-Type: text/plain; charset="utf-8" > > > > > > http://www.agilent.com/cs/library/technicaloverviews/ > > > public/08002_ihc_staining_methods.pdf > > > > > > > > > -----Original Message----- > > > From: Rene J Buesa via Histonet [mailto:histonet@lists. > > utsouthwestern.edu] > > > Sent: Thursday, February 09, 2017 10:51 AM > > > To: Jennifer <histot...@dermspec.com>; histonet@lists.utsouthwestern. > edu > > > Subject: Re: [Histonet] hand staining immunos > > > > > > You do not have to "babysit" the procedure for it has well > defined/timed > > > steps. You just need a timer and check the slides when required. As to > > > procedural "dos & donts" try to get a copy of the DAKO IHC manual.Ren? > > > > > > On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet < > > > histonet@lists.utsouthwestern.edu> wrote: > > > > > > > > > Hello, > > > > > > > > > > > > My dermatopathologist wants me to start hand staining immunos for > MITF. > > I > > > would really like some feedback about everyone's experiences/thoughts > > with > > > manually hand staining immunos and especially with MITF. Pros and > cons? I > > > am concerned about the amount of time this will take for a tech to > > babysit > > > the stain and also about the consistency of the quality in the end > > result. > > > Your opinions are much appreciated. > > > > > > > > > > > > Thank you! > > > > > > > > > > > > Jennifer > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE: The information contained in this electronic > > > message is intended solely for the personal and confidential use of the > > > designated recipient(s) named above and may contain information that is > > > protected from disclosure under applicable law. If you are not the > > intended > > > recipient, or the employee or agent responsible for delivering it to > the > > > intended recipient, you are hereby notified that any dissemination, > > > distribution or copying of this transmission is strictly prohibited. If > > you > > > have received this transmission in error, please notify the > transmitting > > > person/department immediately by email or telephone (410) 581-5881 and > > > delete the message without making a copy. > > > > > > ------------------------------ > > > > > > Subject: Digest Footer > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > > > > > End of Histonet Digest, Vol 159, Issue 8 > > > **************************************** > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Caroline Miller (mills) > Director of Histology > 3Scan.com > 415 2187297 <(415)%20218-7297> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet