We got some organisms from the microbiology lab and some fresh lung from
autopsy. When the organisms were ready, we 'stuck' the lung with the
inoculating loop and let them incubate for a few days. Then we grossed and
processed the lung. We were given separate specimens for GMs, AFB, and gram.
It worked great.
If you don't have lung, some liver from the grocery store would work. We let
that sit in the refrigerator for about two weeks, then did touch preps to see
if anything was growing.
It had fungus galore in the touch prep. We used that to teach students how to
stain touch preps with specials.
Toysha
Message: 3
Date: Mon, 24 Jul 2017 09:48:16 -0400
From: Charles Riley <cri...@dpspa.com>
To: Histo List <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Fungus Controls
Message-ID:
<caaqhb10o2jt9ztgcraypbfwe6q7fcbfcq5absfgmuqd_mih...@mail.gmail.com>
Content-Type: text/plain; charset="UTF-8"
Does anyone grow their own fungus control blocks for GMS and AFB stains?
If so would you mind sharing your procedure for doing so. I tried using rotten
oranges but the sample wouldn't hold onto the counterstain.
--
Charles Riley BS HT, HTL(ASCP)CM
Histopathology Coordinator/ Mohs
------------------------------
Message: 4
Date: Mon, 24 Jul 2017 08:11:40 -0700
From: Akemi <akemiat3...@gmail.com>
To: Charles Riley <cri...@dpspa.com>, Histonet
<Histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Fungus Controls
Message-ID: <6e924300-3ba1-4271-9b8a-8e3d9c754...@gmail.com>
Content-Type: text/plain; charset=us-ascii
I've been told by one of my pathologists that blue cheese for a fungus control
would work.
Akemi Allison
Sent from my iPhone
> On Jul 24, 2017, at 6:48 AM, Charles Riley via Histonet
> <histonet@lists.utsouthwestern.edu> wrote:
>
> Does anyone grow their own fungus control blocks for GMS and AFB stains?
>
> If so would you mind sharing your procedure for doing so. I tried
> using rotten oranges but the sample wouldn't hold onto the counterstain.
>
> --
>
> Charles Riley BS HT, HTL(ASCP)CM
>
> Histopathology Coordinator/ Mohs
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
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