Hi Toysha, How long do you let the tissue/microbes incubate and at room temp? When we tried it in the incubator at different times the tissue was too far gone to make it useful. After a few day you still had viable tissue structure? I'm going to try the liver prep. Thank you so much if you are able to help!! E-van
-----Original Message----- From: Mayer,Toysha N via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, July 25, 2017 11:17 AM To: 'histonet@lists.utsouthwestern.edu' <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Fungus controls We got some organisms from the microbiology lab and some fresh lung from autopsy. When the organisms were ready, we 'stuck' the lung with the inoculating loop and let them incubate for a few days. Then we grossed and processed the lung. We were given separate specimens for GMs, AFB, and gram. It worked great. If you don't have lung, some liver from the grocery store would work. We let that sit in the refrigerator for about two weeks, then did touch preps to see if anything was growing. It had fungus galore in the touch prep. We used that to teach students how to stain touch preps with specials. Toysha Message: 3 Date: Mon, 24 Jul 2017 09:48:16 -0400 From: Charles Riley <cri...@dpspa.com> To: Histo List <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Fungus Controls Message-ID: <caaqhb10o2jt9ztgcraypbfwe6q7fcbfcq5absfgmuqd_mih...@mail.gmail.com> Content-Type: text/plain; charset="UTF-8" Does anyone grow their own fungus control blocks for GMS and AFB stains? If so would you mind sharing your procedure for doing so. I tried using rotten oranges but the sample wouldn't hold onto the counterstain. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ------------------------------ Message: 4 Date: Mon, 24 Jul 2017 08:11:40 -0700 From: Akemi <akemiat3...@gmail.com> To: Charles Riley <cri...@dpspa.com>,Histonet <Histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Fungus Controls Message-ID: <6e924300-3ba1-4271-9b8a-8e3d9c754...@gmail.com> Content-Type: text/plain;charset=us-ascii I've been told by one of my pathologists that blue cheese for a fungus control would work. Akemi Allison Sent from my iPhone > On Jul 24, 2017, at 6:48 AM, Charles Riley via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > Does anyone grow their own fungus control blocks for GMS and AFB stains? > > If so would you mind sharing your procedure for doing so. I tried > using rotten oranges but the sample wouldn't hold onto the counterstain. > > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
