The hyaluronidase is digesting out hyaluronic acid, a component found in connective tissue mucins. This reaction is used to differentiate connective tissue mucins from epithelial mucins. Hyaluronic acid and epithelial sialomucins will give the same staining results, both positive for alcian blue pH 2.5 and negative for pH 1.0.
-----Original Message----- From: Hobbs, Carl via Histonet <histonet@lists.utsouthwestern.edu> Sent: Thursday, August 23, 2018 11:32 AM To: histonet <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives? Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide/section and Ab pH1 ( highly sulphated mucins only) on a sequential slide? Sure, instead use Alcian blue CEC method? I will be interested to read other responses to your request. Mucin-illy carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
