Vielen dank, Michael Very kind of you to send this to me.
Alcian blue is indeed an interesting dye I thought that it was no longer manufactured? I am wrong, obviously. I have used the CEC method many years ago when analysing articular cartilages and it is good to see that it is still a valid method to differentiate the various acidic mucins Alles Gute carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ________________________________ From: Dr. Michael Gudo (Morphisto GmbH) <michael.g...@morphisto.de> Sent: 24 August 2018 11:22:55 To: Hobbs, Carl Cc: histonet Subject: Re: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives? Hello Carl, Alcianblue is q quite interesting stain! Maybe this selection of a presentation helps you to find a suitable method. I attach you a PDF-document to this email. The slides are in german, but I think you will understand them anyway. Kind regards Michael Am 23.08.2018 um 20:32 schrieb Hobbs, Carl via Histonet <histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>>: Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide/section and Ab pH1 ( highly sulphated mucins only) on a sequential slide? Sure, instead use Alcian blue CEC method? I will be interested to read other responses to your request. Mucin-illy carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weismüllerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.g...@morphisto.de<mailto:i...@morphisto.de> Internet: http://www.morphisto.de/<https://emea01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.morphisto.de%2F&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C1ba1d9476b684e63735808d609ab976a%7C8370cf1416f34c16b83c724071654356%7C0&sdata=pq042s3UPpkmLVabW2yshvdlZg7q53mm9G2jWIBX%2BqY%3D&reserved=0> Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gemäß § 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
