Paula:"Optimizing" H&E to detect H. piloris is a rout you should not attempt
because H&E cannot be "optimized" for that.Giemsa is one way or, even better,
modified Steiner stain. The only disadvantage modified Steiner has is using
radioactive uranium nitrate, but I developed a procedure where 0.02% aq.
phosphotungstic acid solution can be used instead. IHC will be way more
expensive.
René
On Tuesday, October 9, 2018 2:03 PM, P Sicurello via Histonet
<histonet@lists.utsouthwestern.edu> wrote:
Good Morning Listers,
Has anyone out there optimized an H&E for H. pylori?
Sure we can run a giemsa or and IHC but what fun is that?
Send me your ideas and make the day of our GI pathologists.
Thanks oodles.
Sincerely,
Paula Sicurello, HTL (ASCP)CM
Histotechnology Specialist
UC San Diego Health
9300 Campus Point Drive
La Jolla, CA 92037
(P): 858-249-5610
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