The following might be useful: Tazawa, K., & Tsutsumi, Y. (1998). Effect of prolonged staining with hematoxylin on detecting Helicobacter pyiori in hematoxylin‐eosin‐stained gastric mucosa. Pathology international, 48(6), 448-452.
Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, 10 October 2018 6:46 AM To: P Sicurello; HistoNet Subject: Re: [Histonet] H&E for H. pylori? Paula:"Optimizing" H&E to detect H. piloris is a rout you should not attempt because H&E cannot be "optimized" for that.Giemsa is one way or, even better, modified Steiner stain. The only disadvantage modified Steiner has is using radioactive uranium nitrate, but I developed a procedure where 0.02% aq. phosphotungstic acid solution can be used instead. IHC will be way more expensive. René On Tuesday, October 9, 2018 2:03 PM, P Sicurello via Histonet <histonet@lists.utsouthwestern.edu> wrote: Good Morning Listers, Has anyone out there optimized an H&E for H. pylori? Sure we can run a giemsa or and IHC but what fun is that? Send me your ideas and make the day of our GI pathologists. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
