Hi histonet, For coverslipping Ventana IHC slides I have dish soap, rinse, alcohol 80%, 100%, 100%, 100%, followed by three Clear-Rite 3 instead of xylene. In the past I didn't have this issue, but now I frequently get a lot of residue on the slides after they enter the Clear-Rite.
If I completely dump all 3 containers of Clear-Rite 3 it gets rid of the problem but I'd have to do this every month which is wasteful. If I just dump the first container and move everything up, the problem still remains. Images: https://i.imgur.com/GTQJe2W.jpg https://i.imgur.com/np8B5P2.jpg https://i.imgur.com/SLypUAy.jpg Anyone know what this contamination is and the best way to reduce it? Allan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
