Thanks all, looks like the consensus is that water into the clearing agent causes it and I should change out alcohol more frequently.
Allan On Wed, Oct 31, 2018 at 5:46 PM Allan Wang <alla...@gmail.com> wrote: > Hi histonet, > > For coverslipping Ventana IHC slides I have dish soap, rinse, alcohol 80%, > 100%, 100%, 100%, followed by three Clear-Rite 3 instead of xylene. In the > past I didn't have this issue, but now I frequently get a lot of residue on > the slides after they enter the Clear-Rite. > > If I completely dump all 3 containers of Clear-Rite 3 it gets rid of the > problem but I'd have to do this every month which is wasteful. If I just > dump the first container and move everything up, the problem still remains. > > Images: > https://i.imgur.com/GTQJe2W.jpg > https://i.imgur.com/np8B5P2.jpg > https://i.imgur.com/SLypUAy.jpg > > Anyone know what this contamination is and the best way to reduce it? > > Allan > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet