following. I haven't done frozen stains on GP only FFPE...interested in what others have to say.
Colleen On Tue, Jan 29, 2019 at 1:58 PM Jan Shivers via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Has anyone ever performed IHC on frozen sections of guinea pig tissue? I > am experiencing an enormous amount of bubbling when doing the peroxidase > blocking step, even though I'm only using a 0.3% concentration of H2O2. > And when I say 'enormous', I mean it's like continuous champagne bubbles > rising out of the tissue, even after 20 minutes in the H2O2 solution. > > I can't find anything in the literature that mentions guinea pigs having a > higher peroxidase content in their tissues. > > Thanks for any help that anyone can provide. > > Jan Shivers > Senior Scientist > IHC/Histology Section Manager > Pathology Teaching Program > Veterinary Diagnostic Laboratory > University of Minnesota > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive...@umn.edu > > *Confidentiality Notice: This message, together with any attachments, is > intended only for the use of the individual or entity to which it is > addressed and may contain confidential or privileged information. If you > think you have received this message in error, please advise the sender and > then delete this message and any attachments immediately.* > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 *If submitting histology request please also forward to Lori Holm at ho...@umn.edu <ho...@umn.edu>* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
