Mary Ann, First I would call the vendor of the antibodies and detection kits and ask them if they have any data about their reagents being warmed up for those time periods. If they can't provide an answer, then I suggest testing every single one of the antibodies and detection kits to see if they still work. I would do something like a mini-validation. Another thing you can do is a literature search that demonstrates the efficacy of warmed up, or old, antibodies/detection kits.
Tally up the cost of replacing all the warmed up reagents. Then calculate the cost of running a test that fails due to the warmed reagents - tech time (remember to include whatever percentage the company adds in terms of benefits), reagents used, etc. Also include the effect on the impression of the clients if you need to repeat a test because the first one failed and you were aware of the reason why it failed. Overall, you are better off replacing all of the items that were warmed up. As for the refrigerator, install a temperature tracking system that will notify someone if the temperature goes out of range. If your lab is licensed by any governing agency, let the CFO/Owner know about any rules or regulations that you must follow when it is suspected that a reagent has gone bad, or expires. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Tue, Mar 26, 2019 at 4:15 PM MARY ANN via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Let's say, hypotheticaly, if you discover your fridge with all you > antibodies and detection kits were discovered to have been at 19c. for an > undisclosed amout of time. 12 24 48 hours due tona power surge..south > Florida weather. > > Let's also propose your lab CFO/Owner dosent think its a big deal. > > First how would you handle the issue given the frisge has been restored ? > > > > > Sent from Xfinity Connect Application > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet