Beth-
I am proud of you for stepping up and correcting the record.
This could not be an easy thing to do in such a public forum, but it also
essential.
I hope you have a wonderful holiday.
Joe Saby
On Wednesday, July 3, 2019, 2:33:16 PM EDT, O'Neil, Beth via Histonet
<[email protected]> wrote:
Last week I posted an inquiry about having problems with Cell Marque's HSV-2
polyclonal antibody. I have to retract my statement that it was Cell Marque
using a different antibody source. After spending two weeks troubleshooting my
stainer, troubleshooting the positive control slides, yelling at my Cell Marque
rep, etc. I found out that it was the positive control slide and not the
antibody. When I originally suspected the positive QC slides (from Cancer
Diagnostics), I requested a different lot number. This new lot also failed to
show positive staining. Long story short, another call to Cancer Diagnostics
finally resulted in receiving confirmation that they are having problems with
their HSV-2 control slides. It was my misfortune to have opened a new box of
QC slides at the same time as opening a new vial of HSV-2 antibody which
resulted in two weeks of headaches. I will say that Cell Marque/Millipore was
very supportive through this. So, for those of you who are using Cancer
Diagnostics HSV-2 control slides, they are working on a resolution and will
replace their "bad" slides. I was told that the staining is "hit or miss, "
hopefully you all have the "hit" slides.
Beth Oneil, WVU Medicine
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