Bravo Beth

Sent from my iPhone

> On Jul 5, 2019, at 11:55 AM, Sheeder, Christopher via Histonet 
> <[email protected]> wrote:
> 
> Beth,
> You have honor and integrity. Something seldom seen these days.
> 
> Chris Sheeder
> Seattle Children's Hospital
> 
> -----Original Message-----
> From: O'Neil, Beth <[email protected]>
> Sent: Wednesday, July 03, 2019 11:20 AM
> To: [email protected]
> Subject: [Histonet] HSV-2 antibody - Cell Marque was not the problem
> 
> Last week I posted an inquiry about having problems with Cell Marque's HSV-2 
> polyclonal antibody.  I have to retract my statement that it was Cell Marque 
> using a different antibody source.   After spending two weeks troubleshooting 
> my stainer, troubleshooting the positive control slides, yelling at my Cell 
> Marque rep, etc.  I found out that it was the positive control slide and not 
> the antibody.  When I originally suspected the positive QC slides (from 
> Cancer Diagnostics), I requested a different lot number.  This new lot also 
> failed to show positive staining.  Long story short, another call to Cancer 
> Diagnostics finally resulted in receiving confirmation that they are having 
> problems with their HSV-2 control slides.  It was my misfortune to have 
> opened a new box of QC slides at the same time as opening a new vial of HSV-2 
> antibody which resulted in two weeks of headaches.  I will say that Cell 
> Marque/Millipore was very supportive through this.  So, for those of you who 
> are using Cancer Diagnostics HSV-2 control slides, they are working on a 
> resolution and will replace their "bad" slides.  I was told that the staining 
> is "hit or miss, "  hopefully you all have the "hit" slides.
> 
> Beth Oneil, WVU Medicine
> 
> 
> 
> 
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