My question is why would you ever want to use an aerosol in the cryostat, 
taking a chance on aerosolizing some nasty bug?
Is the 30 seconds you save in using freezing spray worth the exposure to TB or 
goodness-knows-what?  
We use a simple metal heat extractor (comes standard in our Leicas) and place 
it on top of the freezing block to accelerate the process.  We never have 
issues with turn around times.
For me, no freezing spray.  Ever.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

-----Original Message-----
From: histonet-requ...@lists.utsouthwestern.edu 
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Sent: Wednesday, September 25, 2019 1:00 PM
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Subject: Histonet Digest, Vol 190, Issue 18

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Today's Topics:

   1. Flammable Sprays in Cryostats (Knutson, Deanne)
   2. Re: Flammable Sprays in Cryostats (Akemi)
   3. Re: Flammable Sprays in Cryostats (Hannen, Valerie)
   4. Re: Flammable Sprays in Cryostats (Ingles Claire)


----------------------------------------------------------------------

Message: 1
Date: Wed, 25 Sep 2019 07:50:44 -0500
From: "Knutson, Deanne" <dknut...@primecare.org>
To: "'histonet@lists.utsouthwestern.edu'"
        <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Flammable Sprays in Cryostats
Message-ID:
        
<1e0e2b14c709174b8ac2be0ae7f768330159b5756...@exchange2k7.staprimecare.org>
        
Content-Type: text/plain; charset="us-ascii"

I just received a letter from Leica Biosystems where they are prohibiting the 
usage of flammable
freezing sprays in their cryostats.  What are others using in their cryostats 
to instantly freeze specimens?

Thank you.


Deanne Knutson
Supervisor
Anatomic Pathology
dknut...@primecare.org<mailto:dknut...@primecare.org>






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Message: 2
Date: Wed, 25 Sep 2019 09:05:20 -0500
From: Akemi <akemiat3...@gmail.com>
To: "Knutson, Deanne" <dknut...@primecare.org>, Histonet
        <Histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Flammable Sprays in Cryostats
Message-ID: <19db25eb-9944-406b-917c-0878d6f15...@gmail.com>
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We are purchasing two of their newer UV cryostats and they were adamant that we 
use a non flammable freezing spray because it can damage the UV unit and have 
potential for exploding.

Akemi Allison

Sent from my iPhone

> On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet 
> <histonet@lists.utsouthwestern.edu> wrote:
> 
> I just received a letter from Leica Biosystems where they are prohibiting the 
> usage of flammable
> freezing sprays in their cryostats.  What are others using in their cryostats 
> to instantly freeze specimens?
> 
> Thank you.
> 
> 
> Deanne Knutson
> Supervisor
> Anatomic Pathology
> dknut...@primecare.org<mailto:dknut...@primecare.org>
> 
> 
> 
> 
> 
> 
>  ________________________________
> This email may include confidential and privileged information. If this is 
> not intended for your use, please destroy immediately and contact the sender 
> of the message.
> 
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> privileged. If you are not the intended recipient, notify the sender at once 
> and delete this message completely from your information system. Further use, 
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> privilege or other confidentiality protections.
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------------------------------

Message: 3
Date: Wed, 25 Sep 2019 15:49:00 +0000
From: "Hannen, Valerie" <valerie.han...@parrishmed.com>
To: "'Knutson, Deanne'" <dknut...@primecare.org>
Cc: "Histonet@lists.utsouthwestern.edu"
        <Histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Flammable Sprays in Cryostats
Message-ID:
        <68e18a8b516a49d4b033de54e175687d@PMSVRLEX02.parrishmed.local>
Content-Type: text/plain; charset="us-ascii"

We received the same letter months ago.  We changed to a non-flammable spray.  
It is called STATFREEZE, we purchase it from STATLAB.  The catalog number is 
SL202/134a.  Be careful if you order it because they also sell a product called 
STATFREEZE that is flammable. 



Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com



-----Original Message-----
From: Knutson, Deanne via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, September 25, 2019 8:51 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [EXTERNAL Sender] [Histonet] Flammable Sprays in Cryostats


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Begin Original Message:

----------------------------------------------------------------------
I just received a letter from Leica Biosystems where they are prohibiting the 
usage of flammable

freezing sprays in their cryostats.  What are others using in their cryostats 
to instantly freeze specimens?



Thank you.





Deanne Knutson

Supervisor

Anatomic Pathology

dknut...@primecare.org<mailto:dknut...@primecare.org>













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------------------------------

Message: 4
Date: Wed, 25 Sep 2019 16:42:30 +0000
From: Ingles Claire <cing...@uwhealth.org>
To: "'histonet@lists.utsouthwestern.edu'"
        <Histonet@lists.utsouthwestern.edu>,    "Knutson, Deanne"
        <dknut...@primecare.org>
Subject: Re: [Histonet] Flammable Sprays in Cryostats
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Liquid Nitrogen guns.
Claire
________________________________
From: Knutson, Deanne via Histonet <histonet@lists.utsouthwestern.edu>
Sent: Wednesday, September 25, 2019 7:50 AM
To: 'histonet@lists.utsouthwestern.edu' <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Flammable Sprays in Cryostats

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I just received a letter from Leica Biosystems where they are prohibiting the 
usage of flammable
freezing sprays in their cryostats.  What are others using in their cryostats 
to instantly freeze specimens?

Thank you.


Deanne Knutson
Supervisor
Anatomic Pathology
dknut...@primecare.org<mailto:dknut...@primecare.org>






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