Hi, When I place my sections on the waterbath the paraffin pulls away from it. Leaving just the edge of the tissue. It is very weird. I have tried at different temps . The tissue itself is fine so I do not believe it is the processing. I use the same paraffin for processing as well as embedding. I use Fisher Histoplast IM. I was considering melting a block or two down and embedding them in another paraffin from another lab. Suggestions?
Betsy Molinari HT, ASCP Texas Heart Institute 6770 Bertner Ave. O-511 Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: bmolin...@texasheart.org texasheart.org<https://www.texasheart.org/> | facebook<https://www.facebook.com/Texas.Heart.Institute> | twitter<https://twitter.com/Texas_Heart> This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
