Are the tissues taken directly from the molten wax and placed in the wax-containing mold or are they allowed to cool before embedding?
One cause of tissue separation is the difference in temperature between tissue and embedding wax. Try taking directly from molten wax and embedding directly in the wax-filled mold. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Betsy Molinari via Histonet <histonet@lists.utsouthwestern.edu> Sent: Saturday, 26 October 2019 00:49 To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] waterbath/paraffin Hi, When I place my sections on the waterbath the paraffin pulls away from it. Leaving just the edge of the tissue. It is very weird. I have tried at different temps . The tissue itself is fine so I do not believe it is the processing. I use the same paraffin for processing as well as embedding. I use Fisher Histoplast IM. I was considering melting a block or two down and embedding them in another paraffin from another lab. Suggestions? Betsy Molinari HT, ASCP Texas Heart Institute 6770 Bertner Ave. O-511 Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: bmolin...@texasheart.org texasheart.org<https://www.texasheart.org/> | facebook<https://www.facebook.com/Texas.Heart.Institute> | twitter<https://twitter.com/Texas_Heart> This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet