I agree with Joe. We used to use ETOH for cell blocks, but stopped using it when we started doing IHC biomarker testing on these specimens. Alcohol is good for some proteomic targets, but can be a disaster for others. We also fix all of our cell block specimens that are collected in saline or RPMI in formalin.
Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) richard.car...@hhchealth.org -----Original Message----- From: Joe W. Walker, Jr. via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Friday, October 25, 2019 4:36 PM To: Charles Riley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cell block processing EXTERNAL email is from outside HHC. DO NOT open attachments or click links from unknown senders. As a cytotech, that wouldn’t be my first choice for collections and FNA specimens. The main reason is that once fixed in 95% ETOH you are limited if you need to perform IHC stains on the cell block unless you have validated your IHCs on ETOH fixed specimens. How do you process the FNA rinses that in in ETOH: Only Cell blocks or do you have another cytology liquid prep? Without knowing your prep process, I’d suggest collecting the FNA needle rinses in Hank’s Balanced Salt solution. After making the cell block, you could then formalin fix them. I can send you a procedure that we utilize for this process. The cell blocks cut great, look great, and you can perform IHC an molecular testing if needed. Joe Walker From: Charles Riley <cri...@dpspa.com> Sent: Friday, October 25, 2019 12:57 PM To: Joe W. Walker, Jr. <jwwal...@rrmc.org> Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cell block processing [External Email] This email originated from outside of the organization. Think before you click: Don’t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don’t recognize the sender. Our tech said they use 95% alcohol to collect the specimen. On Fri, Oct 25, 2019 at 12:23 PM Joe W. Walker, Jr. <jwwal...@rrmc.org<mailto:jwwal...@rrmc.org>> wrote: Hi Charles, What are you collecting the FNA into? Cytorich? Cytolyt? Other? Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager joewal...@rrmc.org<mailto:joewal...@rrmc.org>, https://urldefense.proofpoint.com/v2/url?u=http-3A__www.rrmc.org&d=DwIGaQ&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=2CdTK8PmO1-CXFcs6R-WWhnxgbCm1AHZ9wJ_1YOqDoA&s=JIavq_Wfz4S4TppCsMv8kQfnmD9wLQhRVNqSm1eJAO4&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__nam02.safelinks.protection.outlook.com_-3Furl-3Dhttp-253A-252F-252Fwww.rrmc.org-26data-3D02-257C01-257Cjwwalker-2540rrmc.org-257C2403b15e19aa486985ac08d7596c3f45-257C0e55647d438e4a448437e959c3cf2240-257C0-257C0-257C637076193772807309-26sdata-3DVxp3O6jX4PVU4FDmXfFTwIQFG0oR03V3csN45z012Mg-253D-26reserved-3D0&d=DwIGaQ&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=2CdTK8PmO1-CXFcs6R-WWhnxgbCm1AHZ9wJ_1YOqDoA&s=Vn_yzz1gfScLUDaT695jKQgsKf5KhNHP41Y1ZT_Vz-Y&e= > -----Original Message----- From: Charles Riley via Histonet <histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> Sent: Friday, October 25, 2019 8:13 AM To: Histo List <histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> Subject: [Histonet] Cell block processing [External Email] This email originated from outside of the organization. Think before you click: Don’t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don’t recognize the sender. Does anyone have any tips or suggestions on how to better process extremely bloody FNA specimens? 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