Do you float and pick up your sections from a water bath of distilled water with no adhesive added? If not, doing that will help.
John On Wed, Feb 26, 2020 at 6:41 AM, Charles Riley via Histonet <histonet@lists.utsouthwestern.edu> wrote: > Our pathologists are complaining that chunks of the dermis are missing from > IHC slides yet the entire section is present prior to staining. > > Does anyone have any ideas what could cause the tissue to not adhere to the > slides throughout the staining process? We use the Leica Bond stainers. > > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet