Hi Charles, It adhesion problems can arise from several sources, some have already been mentioned. Here are two that I have experienced: 1) Bad slides. Either a manufacturing defect such that the positive charge is insufficient or the slides were somehow compromised after opening them on the bench (perhaps exposed to moisture thus removing the charge). Sometimes a new lot number be better but sometimes switching brands is the quickest fix. 2) Ensuring all water is removed from under the section. I find that with some charged slides (many in fact), water is trapped under the section and does not drain out on standing and does not drain or evaporate when baking at 60C. If the slide with trapped water is put on the instrument with trapped water, the dewax step will lift much of the tissue. Cheers, -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0
*Everything in moderation...even moderation itself**!* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet