I agree, Slightly thicker makes the polarisation easier to see (personal experience).
I would love to see a study comparing section thickness Vs polarisation characteristics. Anyone interested? (being a kids hospital we rarely see amyloidosis) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Paula Keene Pierce via Histonet <histonet@lists.utsouthwestern.edu> Sent: Friday, 17 April 2020 06:24 To: histonet@lists.utsouthwestern.edu; Ken M Subject: Re: [Histonet] Recommended thickness of Amyloid sections As this is my 41 year of being a registered HT, I was taught that slides for amyloid are to be cut at 8-10µm. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Thursday, April 16, 2020, 03:18:44 PM CDT, Ken M via Histonet <histonet@lists.utsouthwestern.edu> wrote: Hello All. We have always cut all of our histology control slides at 5m. We were told today that it is common practice to cut Amyloid at 8m? Is this your experience? Ken _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
