Yes, definitely ice crystal holes! If the tissues are unfixed you will have to freeze much more rapidly (isopentane cooled with liquid nitrogen.) If fixed in formaldehyde, cryoprotect by immersing the pieces in 20% sucrose, until they sink.
John Kiernan Anatomy & Cell Biology, UWO London, Canada = = = ________________________________ From: Bonello Dorianne M at Health-MDH via Histonet <histonet@lists.utsouthwestern.edu> Sent: July 16, 2021 11:25 AM To: histonet@lists.utsouthwestern.edu <histonet@lists.utsouthwestern.edu> Subject: [Histonet] frozen section problem Dear all, We are experiencing freezing artifacts on our frozen sections. Basically, we are seeing cavity-like structures under the microscope, mostly elongated, especially when it's a frozen section on brain tissue. This is most probably happening due to ice crystal formation. We're not using cryospray, relying only on the cryobar boost function. Does anyone has a solution to this problem please? Regards, Dorianne Bonello Allied Health Practitioner (MLS) Histology Laboratory - Pathology Health-Mater Dei Hospital [cid:image001.jpg@01D67184.63288530] T +356 +356 25456434 E dorianne.m.bone...@gov.mt Mater Dei Hospital, Triq id-Donaturi tad-Demm, l-Imsida, Malta MSD 2090 | Tel +356 2545 0000 | https://deputyprimeminister.gov.mt/en/MDH/Pages/Home.aspx<https://deputyprimeminister.gov.mt/en/MDH/Pages/Home.aspx> | https://www.facebook.com/materdeihospital/ Think before you print. Kindly consider your environmental responsibility. This email and any files transmitted with it are confidential, may be legally privileged and intended solely for the use of the individual or entity to whom they are addressed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet