Hi Dorianne,
You need to freeze your tissue faster. Ideally, isopentane placed in a
metal cup, that is that is then frozen in a dewar of liquid nitrogen, works
best. The isopentane, once frozen, is thawed a little with a metal rod to
produce a small liquid pool and your tissue is placed in this for about one
minute. You need some equipment for this procedure, such as the metal cup that
can sit inside a small, open dewar of liquid nitrogen. Alternatively, you can
freeze directly in liquid nitrogen, though you need to beware of the tissue
fracturing due to the sudden and extreme temperature reduction. Slower
freezing of tissue (sitting on dry ice, etc.) allows ice crystals to form in
the tissue, creating the vacuoles you describe.
I hope this helps.
Phil.
Philip Manfre, BA, HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP81-406
770 Sumneytown Pike
West Point, PA 19486
215-652-9750
philip_man...@merck.com
-----Original Message-----
From: Bonello Dorianne M at Health-MDH via Histonet
<histonet@lists.utsouthwestern.edu>
Sent: Friday, July 16, 2021 11:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] frozen section problem
EXTERNAL EMAIL – Use caution with any links or file attachments.
Dear all,
We are experiencing freezing artifacts on our frozen sections. Basically, we
are seeing cavity-like structures under the microscope, mostly elongated,
especially when it's a frozen section on brain tissue. This is most probably
happening due to ice crystal formation. We're not using cryospray, relying only
on the cryobar boost function.
Does anyone has a solution to this problem please?
Regards,
Dorianne Bonello
Allied Health Practitioner (MLS)
Histology Laboratory - Pathology
Health-Mater Dei Hospital
[cid:image001.jpg@01D67184.63288530]
T +356 +356 25456434
E dorianne.m.bone...@gov.mt
Mater Dei Hospital, Triq id-Donaturi tad-Demm, l-Imsida, Malta MSD 2090 | Tel
+356 2545 0000 |
https://deputyprimeminister.gov.mt/en/MDH/Pages/Home.aspx<https://deputyprimeminister.gov.mt/en/MDH/Pages/Home.aspx>
| https://www.facebook.com/materdeihospital/
Think before you print. Kindly consider your environmental responsibility.
This email and any files transmitted with it are confidential, may be legally
privileged and intended solely
for the use of the individual or entity to whom they are addressed.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice: This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
