Hello experts! I would like to find out what others have done to validate a new cryostat. I believe it would basically be the same as validating a new microtome except specimens (ie blocks) are plentiful for microtomy whereas for us, fresh tissue for cryosectioning is not readily available.
Background: We are an acute care hospital and we only get about 50 intraoperative consult requests per year. Our older unit is still functional so I can do side-by-side comparisons with the new unit. Our frozens are only stained for H&E and a very rare (one every couple of years) Oil Red O. So I have two questions: 1. Can I use formalin-fixed tissue soaked overnight in a saturated sucrose solution for the validation? This seems reasonable since I can do side-by-side with the older unit. {autopsy tissue could be an option if the timing coincided with our validation but Murphy's Law will probably prevent this option! lol} 2. How many tissues would be satisfactory? Is one specimen of each of our typical frozen specimen types enough? We typically get: ovary, thyroid, skin and lymph node. Any other tissue type would be rare. I look forward to hearing from you! Thank you, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet