Hi Greg, I would just grab some kidneys from the butcher, freeze and section as usual. They should give equivalent results to the older unit.
Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Greg Dobbin via Histonet <histonet@lists.utsouthwestern.edu> Sent: Monday, 23 August 2021 22:16 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New cryostat validation procedure Hello experts! I would like to find out what others have done to validate a new cryostat. I believe it would basically be the same as validating a new microtome except specimens (ie blocks) are plentiful for microtomy whereas for us, fresh tissue for cryosectioning is not readily available. Background: We are an acute care hospital and we only get about 50 intraoperative consult requests per year. Our older unit is still functional so I can do side-by-side comparisons with the new unit. Our frozens are only stained for H&E and a very rare (one every couple of years) Oil Red O. So I have two questions: 1. Can I use formalin-fixed tissue soaked overnight in a saturated sucrose solution for the validation? This seems reasonable since I can do side-by-side with the older unit. {autopsy tissue could be an option if the timing coincided with our validation but Murphy's Law will probably prevent this option! lol} 2. How many tissues would be satisfactory? Is one specimen of each of our typical frozen specimen types enough? We typically get: ovary, thyroid, skin and lymph node. Any other tissue type would be rare. I look forward to hearing from you! Thank you, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet