Cryo-electron microscopy cuts thin sections at temperatures colder than that. It is used to preserve antigenicity in certain situations.
However, a cryostat is not used. An ultramicrotome fitted with specific cryo attachments is used. Sincerely, Paula Sicurello Sent from my iPhone > On May 18, 2022, at 12:55 PM, Colleen Forster via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > Following....I am in research and I have never cut this cold. > > Colleen Forster > >> On Wed, May 18, 2022 at 2:04 PM Ken Marzinsky via Histonet < >> histonet@lists.utsouthwestern.edu> wrote: >> >> Can anyone tell me why research labs often require cryostat cutting head >> temperatures down to -60C? >> >> Ken >> Durham, NC >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > -- > Colleen Forster HT(ASCP)QIHC > BLS Histology and IHC Laboratory > Jackson Hall, Room 2-155 > 321 Church St. SE > Minneapolis, MN 55455 > 612-626-1930 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
