I have used down to -30 a few times for research samples, mainly to try and get stuff to cut better rather than that is what was requested. Everything gets very brittle at that temperature, including your hands after a short while.
Never -60 though! Maybe that is just for the peltier freezing mechanism? For putting the tissue on the chucks with OCT glue? I typically will set the specimen head a little lower than the chamber. But only 3-4 degrees This is the one I typically use and it is -40 max: https://www.leicabiosystems.com/us/histology-equipment/cryostats/leica-cm3050-s/ I would be interested to know if anyone works at -60 in the LM world! Caroline On Thu, May 19, 2022 at 12:37 PM Colleen Forster via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Thank you Paul. > > Colleen > > On Thursday, May 19, 2022, P Sicurello <pat...@gmail.com> wrote: > > > Cryo-electron microscopy cuts thin sections at temperatures colder than > > that. It is used to preserve antigenicity in certain situations. > > > > However, a cryostat is not used. An ultramicrotome fitted with specific > > cryo attachments is used. > > > > Sincerely, > > > > Paula Sicurello > > > > Sent from my iPhone > > > > > On May 18, 2022, at 12:55 PM, Colleen Forster via Histonet < > > histonet@lists.utsouthwestern.edu> wrote: > > > > > > Following....I am in research and I have never cut this cold. > > > > > > Colleen Forster > > > > > >> On Wed, May 18, 2022 at 2:04 PM Ken Marzinsky via Histonet < > > >> histonet@lists.utsouthwestern.edu> wrote: > > >> > > >> Can anyone tell me why research labs often require cryostat cutting > head > > >> temperatures down to -60C? > > >> > > >> Ken > > >> Durham, NC > > >> _______________________________________________ > > >> Histonet mailing list > > >> Histonet@lists.utsouthwestern.edu > > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >> > > > > > > > > > -- > > > Colleen Forster HT(ASCP)QIHC > > > BLS Histology and IHC Laboratory > > > Jackson Hall, Room 2-155 > > > 321 Church St. SE > > > Minneapolis, MN 55455 > > > 612-626-1930 > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > -- > Colleen Forster HT(ASCP)QIHC > BLS Histology and IHC Laboratory > Jackson Hall, Room 2-155 > 321 Church St. SE > Minneapolis, MN 55455 > 612-626-1930 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller, M.Sc Histology, Imaging, and Image analysis consultant 415 2187297 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
