What species are you staining?? Amy S. Porter, HT (ASCP) - Lab Supervisor Michigan State University Departments of Physiology & Pathology Investigative HistoPathology Lab 567 Wilson Road - Rm 2133 East Lansing, MI 48824 Ph: 517-884-5026 (she/her/hers) https://sites.google.com/msu.edu/ihpl/home "Life is like riding a bicycle. To keep your balance, you must keep moving." - Albert Einstein
-----Original Message----- From: Charles Riley via Histonet <histonet@lists.utsouthwestern.edu> Sent: Thursday, June 23, 2022 1:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Active Caspase-3 IHC-P staining I am currently running a research project and the researcher wants me to use Caspase-3 for apoptosis staining. I have never worked with this antibody before and want to know if anyone has any suggestions on what to use for a positive tissue control and a negative tissue control? I purchased the antibody from Novus Biologicals and they recommend a Jurkat cell lysate for positive control but I am not familiar with this as well. Any guidance would be greatly appreciated _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!xyBsRdDpZSchS-atSTL78qekJGvTEx9dKu9K1y7IVA7l5o-LPaWNWLRv2PASuqtgcrm-OQLBRf04blsJq4zdxucLZzY39A$ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
