If you have access to tissues, rather than purchasing cell lines, you can use spleen, gut, lymph nodes. They will all have a lot of apoptosis. You can use a lot of different tissues for negative controls: I prefer brain or muscle since they are less dense with nuclei.
Val > On Jun 23, 2022, at 10:32 AM, Charles Riley via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > I am currently running a research project and the researcher wants me to > use Caspase-3 for apoptosis staining. I have never worked with this > antibody before and want to know if anyone has any suggestions on what to > use for a positive tissue control and a negative tissue control? > > > I purchased the antibody from Novus Biologicals and they recommend a Jurkat > cell lysate for positive control but I am not familiar with this as well. > > Any guidance would be greatly appreciated > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
