Very well stated! I too have been histology for 3 decades an never ever heard of cold mold embedding. Unbelievable!! Thank you Jay for your insight… Steven Mello, BS HT(ASCP)
Sent from my iPhone > On Sep 10, 2022, at 5:47 PM, Shirley Ennis via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > Jay ,I totally agree . > > Shirley > > Get Outlook for iOS<https://aka.ms/o0ukef> > ________________________________ > From: Shirley A. Powell via Histonet <histonet@lists.utsouthwestern.edu> > Sent: Saturday, September 10, 2022 8:12:35 AM > To: Jay Lundgren <jaylundg...@gmail.com>; Naira Margaryan > <nmargarya...@gmail.com> > Cc: histonet@lists.utsouthwestern.edu <histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Molds- cold vs warm > > Well said Jay. > Thanks, > Shirley Powell > > > -----Original Message----- > From: Jay Lundgren via Histonet <histonet@lists.utsouthwestern.edu> > Sent: Friday, September 9, 2022 5:37 PM > To: Naira Margaryan <nmargarya...@gmail.com> > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Molds- cold vs warm > > Whoever is telling you to use cold molds needs to go back to clown college. > > That is totally, 100%, absolutely, wrong. > > There is some debate as to embed "wet" (cassettes submerged in paraffin > bath) or "dry", and I will accept either, as mostly a matter of personal > preference. BUT, in both of these cases, the molds are hot. > > I have been a Histotech for five decades, trained at Armed Forces Institute > of Pathology (back when that used to mean something) and I have NEVER seen > anyone using cold molds. > > It is a guaranteed way to get cold fractures and cracks in your blocks, or to > pop tissue out when you are cutting, which might be irretrievable. Just think > how much time all those re-embedded blocks are going to save you! > > Also, you won't be able to easily re-position specimens in the block, to put > them "on edge" or "on end", for example. The tissue will instantly stick to > the cold mold. And if you want to re-position it, guess what, you'll have to > warm the mold up to get the tissue unstuck. How's that (non-existent anyway) > time savings now? > > > If you want to prove to whatever jackass suggested this that they are wrong, > get a big stack of every histopathology textbook you can find. > There is nothing in any of them talking about paraffin embedding with cold > molds. > > As a matter of fact, every single textbook will specify molds at the same > temp as paraffin. > > Anyway, it doesn't even make sense, thermodynamically. Heat travels from hot > to cold. Those "cold" molds will be the same temperature as the paraffin, > almost instantly. Did it take a tiny amount of heat out of the hot paraffin? > Yes, but not enough to noticeably cool the blocks faster. The amount of heat > from the paraffin used to warm the mold is trivial compared to the total heat > of the system. That's why cold plates have huge, noisy refrigeration units. > You can't argue with thermodynamics. > > If you are having trouble getting your blocks to release, use mold release! > Viola! > https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.statlab.com%2F&data=05%7C01%7Cpowell_sa%40mercer.edu%7C84d81a9eddad4ba8159308da92ab72a7%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C0%7C637983562331339740%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=YDxIy7oMkibqOiMnl0D44LCG3XD0oKPY7jFHZBHBrsM%3D&reserved=0. > I used to think it was superfluous, but now I consider it compulsory. This > is probably the answer to most of your issues. > > I don't know who is suggesting using cold molds, but I can pretty much > guarantee that it's a pathologist who thinks his slides are taking too long, > and knows nothing about histopathology, or a lab manager, who knows nothing > about histopathology. This next part is directly to them. > > To Whoever Suggested Cold Molds: The answer to getting your slides out > quicker is buying more equipment and hiring more techs, and holding everyone > to standards (30 blocks/hr cutting, 60 blocks/hr embedding). > Making nonsensical, uninformed suggestions only exposes your ignorance. > > Please feel free to show them this reply. > > Sincerely, > > Jay A. Lundgren, M.S., HTL (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Cpowell_sa%40mercer.edu%7C84d81a9eddad4ba8159308da92ab72a7%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C0%7C637983562331339740%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=zA5yQmG4SG0GCEs7Wqw%2FUegWBgNiTZl5gVC5EaQRMMI%3D&reserved=0 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
