Tell us how you really feel Jay. LOL. You say everything that I would say and have said while training people. Like you having been doing this for a bit (1980) and had the privilege of meeting Mr. Lee Luna.
Sent from Yahoo Mail for iPad On Friday, September 9, 2022, 6:14 PM, Naira Margaryan via Histonet <histonet@lists.utsouthwestern.edu> wrote: Thank You so much Jay, for such detailed explanation and for permission to use your email to address. My sincere regards, Naira On Fri, Sep 9, 2022 at 5:00 PM Cooper, Brian <bcoo...@chla.usc.edu> wrote: > Thanks for saying this Jay!! I have to say, it's been a while since we've > had such a great response on Histonet!! Everything you said is spot on. > > Happy Friday everyone! > > Thanks, > > Brian Cooper > Histology Supervisor > Children's Hospital Los Angeles > Sent from my mobile > > > > > > On Sep 9, 2022 2:37 PM, Jay Lundgren via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** > This email came from outside CHLA. Do not open attachments, click on > links, or respond unless you expected this message and recognize the email > address: histonet-boun...@lists.utsouthwestern.edu. > > > Whoever is telling you to use cold molds needs to go back to clown college. > > That is totally, 100%, absolutely, wrong. > > There is some debate as to embed "wet" (cassettes submerged in paraffin > bath) or "dry", and I will accept either, as mostly a matter of personal > preference. BUT, in both of these cases, the molds are hot. > > I have been a Histotech for five decades, trained at Armed Forces Institute > of Pathology (back when that used to mean something) and I have NEVER seen > anyone using cold molds. > > It is a guaranteed way to get cold fractures and cracks in your blocks, or > to pop tissue out when you are cutting, which might be irretrievable. Just > think how much time all those re-embedded blocks are going to save you! > > Also, you won't be able to easily re-position specimens in the block, to > put them "on edge" or "on end", for example. The tissue will instantly > stick to the cold mold. And if you want to re-position it, guess what, > you'll have to warm the mold up to get the tissue unstuck. How's that > (non-existent anyway) time savings now? > > > If you want to prove to whatever jackass suggested this that they are > wrong, get a big stack of every histopathology textbook you can find. > There is nothing in any of them talking about paraffin embedding with cold > molds. > > As a matter of fact, every single textbook will specify molds at the same > temp as paraffin. > > Anyway, it doesn't even make sense, thermodynamically. Heat travels from > hot to cold. Those "cold" molds will be the same temperature as the > paraffin, almost instantly. Did it take a tiny amount of heat out of the > hot paraffin? Yes, but not enough to noticeably cool the blocks faster. The > amount of heat from the paraffin used to warm the mold is trivial compared > to the total heat of the system. That's why cold plates have huge, noisy > refrigeration units. You can't argue with thermodynamics. > > If you are having trouble getting your blocks to release, use mold > release! Viola! > https://secure-web.cisco.com/1vhENcmRngDgLubdLEYMzzWWUK4ILg_WIJNnMutz67Oikk5LSg5SqF6OvSQqMWpr4MIirbF_ExGbIXm9Usdm35LUk87pXYTIvPVdKY5u2dRCdo_Ss-iuZ4nCOa0nPTIpPec8zwvOBcVIE7eM7o-flt9BAIGK0ZOw4K3HOXwNiLmQBnD0hFb9pgrU0ZuPnk5llOYCeJ5b2Pmkp2B9UPlVvxPMI3-iHRILtOB4kPL45PII_yUJnJhFYAryeid5lrITtm-w0KNyKrfJVI0mHy47Niz0TEpxxvl3DoTDmq-umsyN3BucCj2B-aJFqJ-AW3thtXSEk-Nl0NzBBrSxw8cPzSrKsVww7cCLh_krbh7VXKlRiRGF41o3UKk_oEQuHGIEeYlUNLnpLndnkSH0cwR3nNWhq3Cy8hw6ws0Ka8kYRH8_TVttsOh_lQbO4tm6_i-fdNOZxcR_7t-QeE9aW5YP1hg/https%3A%2F%2Fwww.statlab.com > I used to think it was > superfluous, but now I consider it compulsory. This is probably the answer > to most of your issues. > > I don't know who is suggesting using cold molds, but I can pretty much > guarantee that it's a pathologist who thinks his slides are taking too > long, and knows nothing about histopathology, or a lab manager, who knows > nothing about histopathology. This next part is directly to them. > > To Whoever Suggested Cold Molds: The answer to getting your slides out > quicker is buying more equipment and hiring more techs, and holding > everyone to standards (30 blocks/hr cutting, 60 blocks/hr embedding). > Making nonsensical, uninformed suggestions only exposes your ignorance. > > Please feel free to show them this reply. > > Sincerely, > > Jay A. 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